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dc.contributor.author
Ortega, Francisco G.
dc.contributor.author
Fernández Baldo, Martín Alejandro
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Serrano, María J.
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Messina, Germán Alejandro
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Lorente, José A.
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Raba, Julio
dc.date.available
2018-06-26T17:09:39Z
dc.date.issued
2015-07
dc.identifier.citation
Ortega, Francisco G.; Fernández Baldo, Martín Alejandro; Serrano, María J.; Messina, Germán Alejandro; Lorente, José A.; et al.; Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform; Elsevier Science Sa; Sensors and Actuators B: Chemical; 221; 7-2015; 248-256
dc.identifier.issn
0925-4005
dc.identifier.uri
http://hdl.handle.net/11336/50097
dc.description.abstract
In the present work, we report a microfluidic immunosensor for epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule) determination. It is based on the use of synthetized silver nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were covalently attached to the central channel (CC) of the microfluidic immunosensor. These nanoparticles were employed as platform for anti-EpCAM monoclonal antibodies immobilization for specifically recognize and capture EpCAM in peripheral blood samples. Afterwards, the amount of this trapped epithelial cancer biomarker was quantified by HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of EpCAM in the peripheral blood sample. The structure and morphology of synthetized AgNPs-Cts were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The calculated detection limits for microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg mL-1, respectively and the within- and between-assay coefficients of variation were below 6.37% for the proposed method. The microfluidic immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care clinical diagnosis and prognosis of epithelial origin tumors in biological samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Sa
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Circulating Tumor Cells
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Epcam
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Epithelial Cancer
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Microfluidic Immunosensor
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Platform
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Silver Nanoparticles
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-26T13:45:52Z
dc.journal.volume
221
dc.journal.pagination
248-256
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Ortega, Francisco G.. Universidad de Granada; España
dc.description.fil
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Serrano, María J.. Universidad de Granada; España
dc.description.fil
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Lorente, José A.. Universidad de Granada; España
dc.description.fil
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.journal.title
Sensors and Actuators B: Chemical
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400515008412
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.snb.2015.06.066
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