Artículo
Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
Ortega, Francisco G.; Fernández Baldo, Martín Alejandro
; Serrano, María J.; Messina, Germán Alejandro
; Lorente, José A.; Raba, Julio
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
07/2015
Editorial:
Elsevier Science Sa
Revista:
Sensors and Actuators B: Chemical
ISSN:
0925-4005
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In the present work, we report a microfluidic immunosensor for epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule) determination. It is based on the use of synthetized silver nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were covalently attached to the central channel (CC) of the microfluidic immunosensor. These nanoparticles were employed as platform for anti-EpCAM monoclonal antibodies immobilization for specifically recognize and capture EpCAM in peripheral blood samples. Afterwards, the amount of this trapped epithelial cancer biomarker was quantified by HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of EpCAM in the peripheral blood sample. The structure and morphology of synthetized AgNPs-Cts were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The calculated detection limits for microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg mL-1, respectively and the within- and between-assay coefficients of variation were below 6.37% for the proposed method. The microfluidic immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care clinical diagnosis and prognosis of epithelial origin tumors in biological samples.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Ortega, Francisco G.; Fernández Baldo, Martín Alejandro; Serrano, María J.; Messina, Germán Alejandro; Lorente, José A.; et al.; Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform; Elsevier Science Sa; Sensors and Actuators B: Chemical; 221; 7-2015; 248-256
Compartir
Altmétricas