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dc.contributor.author
Carabelli, Julieta  
dc.contributor.author
Quattrocchi, Valeria  
dc.contributor.author
D'antuono, Alejandra Lorena  
dc.contributor.author
Zamorano, Patricia Ines  
dc.contributor.author
Tribulatti, María Virginia  
dc.contributor.author
Campetella, Oscar Eduardo  
dc.date.available
2018-06-24T15:38:12Z  
dc.date.issued
2017-11  
dc.identifier.citation
Carabelli, Julieta; Quattrocchi, Valeria; D'antuono, Alejandra Lorena; Zamorano, Patricia Ines; Tribulatti, María Virginia; et al.; Galectin-8 activates dendritic cells and stimulates antigen-specific immune response elicitation; Federation of American Societies for Experimental Biology; Journal of Leukocyte Biology; 102; 5; 11-2017; 1237-1247  
dc.identifier.issn
0741-5400  
dc.identifier.uri
http://hdl.handle.net/11336/49887  
dc.description.abstract
Galectin-8 (Gal-8) is a mammalian β-galactoside-binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen-specific immune responses in vivo, we investigated whether Gal-8 was also able to promote APC activation to sustain T cell activation after priming. Both endogenous [dendritic cells (DCs)] and bone marrow-derived DCs (BMDCs) treated with exogenous Gal-8 exhibited a mature phenotype characterized by increased MHC class II (MHCII), CD80, and CD86 surface expression. Moreover, Gal-8-treated BMDCs (Gal-8–BMDCs) stimulated antigen-specific T cells more efficiently than immature BMDCs (iBMDCs). Proinflammatory cytokines IL-3, IL-2, IL-6, TNF, MCP-1, and MCP-5, as well as growth factor G-CSF, were augmented in Gal-8–BMDC conditioned media, with IL-6 as the most prominent. Remarkably, BMDCs from Gal-8-deficient mice (Lgals8-/- BMDC) displayed reduced CD86 and IL-6 expression and an impaired ability to promote antigen-specific CD4 T cell activation. To test if Gal-8-induced activation correlates with the elicitation of an effective immune response, soluble Gal-8 was coadministrated with antigen during immunization of BALB/cJ mice in the experimental foot-and-mouth disease virus (FMDV) model. When a single dose of Gal-8 was added to the antigen formulation, an increased specific and neutralizing humoral response was developed, sufficient to enhance animal protection upon viral challenge. IL-6 and IFN-γ, as well as lymphoproliferative responses, were also incremented in Gal-8/antigen-immunized animals only at 48 h after immunization, suggesting that Gal-8 induces the elicitation of an inflammatory response at an early stage. Taking together, these findings argue in favor of the use of Gal-8 as an immune-stimulator molecule to enhance the adaptive immune response.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Federation of American Societies for Experimental Biology  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Adjuvant  
dc.subject
Foot-And-Mouth Disease Virus  
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Galectin-8 Knock-Out Mice  
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Galectins  
dc.subject
Interleukin-6  
dc.subject.classification
Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Galectin-8 activates dendritic cells and stimulates antigen-specific immune response elicitation  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-22T15:05:46Z  
dc.journal.volume
102  
dc.journal.number
5  
dc.journal.pagination
1237-1247  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Bethesda  
dc.description.fil
Fil: Carabelli, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina  
dc.description.fil
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina  
dc.description.fil
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina  
dc.description.fil
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina  
dc.description.fil
Fil: Tribulatti, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina  
dc.description.fil
Fil: Campetella, Oscar Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina  
dc.journal.title
Journal of Leukocyte Biology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1189/jlb.3A0816-357RR  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://jlb.onlinelibrary.wiley.com/doi/abs/10.1189/jlb.3A0816-357RR