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Artículo

Galectin-8 activates dendritic cells and stimulates antigen-specific immune response elicitation

Carabelli, JulietaIcon ; Quattrocchi, ValeriaIcon ; D'antuono, Alejandra LorenaIcon ; Zamorano, Patricia InesIcon ; Tribulatti, María VirginiaIcon ; Campetella, Oscar EduardoIcon
Fecha de publicación: 11/2017
Editorial: Federation of American Societies for Experimental Biology
Revista: Journal of Leukocyte Biology
ISSN: 0741-5400
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Inmunología

Resumen

Galectin-8 (Gal-8) is a mammalian β-galactoside-binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen-specific immune responses in vivo, we investigated whether Gal-8 was also able to promote APC activation to sustain T cell activation after priming. Both endogenous [dendritic cells (DCs)] and bone marrow-derived DCs (BMDCs) treated with exogenous Gal-8 exhibited a mature phenotype characterized by increased MHC class II (MHCII), CD80, and CD86 surface expression. Moreover, Gal-8-treated BMDCs (Gal-8–BMDCs) stimulated antigen-specific T cells more efficiently than immature BMDCs (iBMDCs). Proinflammatory cytokines IL-3, IL-2, IL-6, TNF, MCP-1, and MCP-5, as well as growth factor G-CSF, were augmented in Gal-8–BMDC conditioned media, with IL-6 as the most prominent. Remarkably, BMDCs from Gal-8-deficient mice (Lgals8-/- BMDC) displayed reduced CD86 and IL-6 expression and an impaired ability to promote antigen-specific CD4 T cell activation. To test if Gal-8-induced activation correlates with the elicitation of an effective immune response, soluble Gal-8 was coadministrated with antigen during immunization of BALB/cJ mice in the experimental foot-and-mouth disease virus (FMDV) model. When a single dose of Gal-8 was added to the antigen formulation, an increased specific and neutralizing humoral response was developed, sufficient to enhance animal protection upon viral challenge. IL-6 and IFN-γ, as well as lymphoproliferative responses, were also incremented in Gal-8/antigen-immunized animals only at 48 h after immunization, suggesting that Gal-8 induces the elicitation of an inflammatory response at an early stage. Taking together, these findings argue in favor of the use of Gal-8 as an immune-stimulator molecule to enhance the adaptive immune response.
Palabras clave: Adjuvant , Foot-And-Mouth Disease Virus , Galectin-8 Knock-Out Mice , Galectins , Interleukin-6
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/49887
DOI: https://dx.doi.org/10.1189/jlb.3A0816-357RR
URL: https://jlb.onlinelibrary.wiley.com/doi/abs/10.1189/jlb.3A0816-357RR
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Carabelli, Julieta; Quattrocchi, Valeria; D'antuono, Alejandra Lorena; Zamorano, Patricia Ines; Tribulatti, María Virginia; et al.; Galectin-8 activates dendritic cells and stimulates antigen-specific immune response elicitation; Federation of American Societies for Experimental Biology; Journal of Leukocyte Biology; 102; 5; 11-2017; 1237-1247
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