Artículo
Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa
Aguilera, Andrea Carolina
; Boschin, Verónica; Carvelli, Flavia Lorena
; Cavicchia, Juan Carlos
; Sosa Escudero, Miguel Angel
Fecha de publicación:
11/2016
Editorial:
Wiley-liss, Div John Wiley & Sons Inc
Revista:
Journal of Cellular Biochemistry
ISSN:
0730-2312
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that β-galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N-acetyl-β-D-glucosaminidase and β-glucuronidase with spermatozoa appeared to be stronger. On the other hand, α-mannosidase and α-fucosidase cannot be removed from the gametes. In addition, part of N-acetyl-β-D-glucosaminidase, β-galactosidase, and β-glucuronidase can also be released by mannose-6-phosphate. Taking into account these data, we explored the presence of cation-independent- and cation-dependent-mannose-6-phosphate receptors in the spermatozoa and found that cation-independent mannose-6-phosphate receptor is highly expressed in bull spermatozoa and cation-dependent-mannose-6-phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation-independent-mannose-6-phosphate receptor is mostly located at the acrosomal zone, whereas cation-dependent-mannose-6-phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N-acetyl-β-D-glucosaminidase and β-glucuronidase isolated from epididymal fluid interacted mostly with cation-independent-mannose-6-phosphate receptor, while β-galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos-6-phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464–2472, 2016.
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Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Aguilera, Andrea Carolina; Boschin, Verónica; Carvelli, Flavia Lorena; Cavicchia, Juan Carlos; Sosa Escudero, Miguel Angel; Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 117; 11-2016; 2464–2472
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