Artículo
Genome analysis of a clinical isolate of Shewanella sp. uncovered an active hybrid integrative and conjugative element carrying an integron platform inserted in a novel genomic locus
Parmeciano Di Noto, Gisela Paula
; Jara, Eugenio; Iriarte Odini, Andrés; Centron, Daniela
; Quiroga, Cecilia



Fecha de publicación:
08/2016
Editorial:
Society for General Microbiology
Revista:
Microbiology-UK
ISSN:
1350-0872
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Resumen
Shewanella spp. are currently considered emerging pathogens that can code for a blaOXA carbapenemase in their chromosome. Complete genome analysis of the clinical isolate Shewanella sp. Sh95 revealed that this strain is a novel species that shares a lineage with marine isolates. Characterization of its resistome showed that it codes for genes drfA15, qacH and blaOXA-48. We propose that Shewanella sp. Sh95 acts as reservoir of blaOXA-48. Moreover, mobilome analysis showed that it contains a novel ICE, named ICESh95. Comparative analysis between the close relatives ICESpuPO1 from Shewanella sp. W3-18-1 and ICE SXTMO10 from Vibrio cholerae showed that ICESh95 encompassed two new regions, a type III restriction modification system and a multidrug resistance integron. The integron platform contained a novel arrangement formed by gene cassettes drfA15 and qacH and a class C-attC group II intron. Furthermore, insertion of ICESh95 occurred at a unique target site, which correlates with the presence of a different xis/int module. Mobility of ICESh95 was assessed showing its ability to self-transfer at high levels to different bacteria species. The presence of a highly adaptable element capable of acquiring antibiotic resistance determinants, combined with its efficient activity to self-transfer makes this element a clear threat for the control of the dissemination of antimicrobial resistance; a troublesome scenario when natural bacteria from water ecosystems, such as Shewanella, act as vector in its propagation.
Archivos asociados
https://dx.doi.org/10.1099/mic.0.000310
http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000310
http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000310

Citación:
Parmeciano Di Noto, Gisela Paula; Jara, Eugenio; Iriarte Odini, Andrés; Centron, Daniela; Quiroga, Cecilia; Genome analysis of a clinical isolate of Shewanella sp. uncovered an active hybrid integrative and conjugative element carrying an integron platform inserted in a novel genomic locus; Society for General Microbiology; Microbiology-UK; 162; 8; 8-2016; 1335-1345
Comunidades y colecciones
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Articulos(IMPAM) [219]
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA