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Artículo

Genetic analysis of a PER-2-producing Shewanella sp. strain harbouring a variety of mobile genetic elements and antibiotic resistance determinants

Almuzara, Marisa; Montaña, Sabrina DaianaIcon ; Lazzaro, Terese; Uong, Sylvia; Parmeciano Di Noto, Gisela PaulaIcon ; Traglia, German MatiasIcon ; Bakai, Romina; Centron, DanielaIcon ; Iriarte Odini, Andrés; Quiroga, CeciliaIcon ; Ramirez, Maria SoledadIcon
Fecha de publicación: 12/2017
Editorial: Elsevier
Revista: Journal of Global Antimicrobial Resistance
ISSN: 2213-7165
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The objective of this study was to investigate the molecular mechanisms explaining the multidrug-resistant (MDR) phenotype found in a novel clinical Shewanella sp. strain (Shew256) recovered from a diabetic patient. Whole-genome shotgun sequencing was performed using Illumina MiSeq-I and Nextera XT DNA library. De novo assembly was performed with SPAdes. RAST Server was used to predict the open-reading frames and the predictions were confirmed using BLAST. Further genomic analysis was carried out using average nucleotide identity (ANI), ACT (Artemis), OrthoMCL, ARG-ANNOT, ISfinder, PHAST, tRNAscan-SE, plasmidSPAdes, PlasmidFinder and MAUVE. PCR and plasmid extraction were also performed. Genomic analysis revealed a total of 456 predicted genes unique to Shew256 compared with other Shewanella genomes. Moreover, the presence of different resistance genes, including blaPER-2, was found. A complex class 1 integron containing the ISCR1 gene, disrupted by two putative transposase genes, was identified. Furthermore, other resistance genes, a transposon containing aph(3′), insertion sequences, phages and non-coding RNAs were also found. In conclusion, evidence of acquisition of resistance genes and mobile elements that could explain the MDR phenotype were observed. This Shewanella sp. represents a prime example of how antibiotic resistance determinants can be acquired by uncommon pathogens.
Palabras clave: Shewanella , Draft Genome , Bla Per-2 , Antimicrobial Resistance
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/47514
DOI: https://dx.doi.org/10.1016/j.jgar.2017.06.005
URL: https://www.sciencedirect.com/science/article/pii/S2213716517301170
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Almuzara, Marisa; Montaña, Sabrina Daiana; Lazzaro, Terese; Uong, Sylvia; Parmeciano Di Noto, Gisela Paula; et al.; Genetic analysis of a PER-2-producing Shewanella sp. strain harbouring a variety of mobile genetic elements and antibiotic resistance determinants; Elsevier; Journal of Global Antimicrobial Resistance; 11; 12-2017; 81-86
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