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Artículo

Measurement of drug-stabilized Topoisomerase II cleavage complexes by Flow Cytometry

de Campos Nebel, Ildefonso MarceloIcon ; Palmitelli, Micaela; Gonzalez Cid, Marcela BeatrizIcon
Fecha de publicación: 07/2017
Editorial: Wiley
Revista: Current protocols in cytometry
ISSN: 1934-9297
e-ISSN: 1934-9300
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

The poisoning of Topoisomerase II (Top2) has been found to be useful as a therapeutic strategy for the treatment of several tumors. The mechanisms of these agents involves a drug-mediated stabilization of a Top2-DNA complex, termed Top2 cleavage complex (Top2cc), which maintains a 5´ end of DNA covalently bound to a tyrosine from Top2 through a phosphodiester group. Drug-stabilized Top2cc leads to Top2 linked-DNA breaks which are believed to mediate their cytotoxicity. Several time-consuming or cell type-limiting assays have been used in the past to study drug-stabilized Top2cc. Here, we describe a flow cytometry-based method that allows arapid assessment of drug-induced Top2cc, which is suitable for high throughput analysis in almost any kind of human cell. The analyses of the drug-induced Top2cc in the cell cycle context and the possibility to track its removal are additional benefits from this methodology.
Palabras clave: Topoisomerase Ii , Cleavage Complexes , High-Throughput Analysis , Human Cells , Dna Topoisomerases , Citometry
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/45036
URL: https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpcy.21
DOI: http://dx.doi.org/10.1002/cpcy.21
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Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
de Campos Nebel, Ildefonso Marcelo; Palmitelli, Micaela; Gonzalez Cid, Marcela Beatriz; Measurement of drug-stabilized Topoisomerase II cleavage complexes by Flow Cytometry; Wiley; Current protocols in cytometry; 81; 7; 7-2017; 7.48.1-7.48.8
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