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dc.contributor.author
Meringer, Maria Veronica  
dc.contributor.author
Villasuso, Ana Laura  
dc.contributor.author
Pasquaré, Susana Juana  
dc.contributor.author
Giusto, Norma Maria  
dc.contributor.author
Machado, Estela Edelmira  
dc.contributor.author
Racagni, Graciela Esther  
dc.date.available
2018-05-11T13:44:00Z  
dc.date.issued
2012-06-23  
dc.identifier.citation
Meringer, Maria Veronica; Villasuso, Ana Laura; Pasquaré, Susana Juana; Giusto, Norma Maria; Machado, Estela Edelmira; et al.; Comparative phytohormone pro les, lipid kinase and lipid phosphatase activities in barley aleurone, coleoptile, and root tissues; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 58; 23-6-2012; 83-88  
dc.identifier.issn
0981-9428  
dc.identifier.uri
http://hdl.handle.net/11336/44876  
dc.description.abstract
We analyzed lipid kinase and lipid phosphatase activities and determined endogenous phytohormone levels by liquid chromatography–tandem mass spectrometry in root and coleoptile tissues following germination of barley (Hordeum vulgare) seeds. The enzymes showing highest activity in aleurone cells were diacylglycerol kinase (DAG-k, EC 2.7.1.107) and phosphatidate kinase (PA-k). The ratio of gibberellins (GAs) to abscisic acid (ABA) was 2-fold higher in aleurone than in coleoptile or root tissues. In coleoptiles, phosphatidylinositol 4-kinase (PI4-k, EC 2.7.1.67) showed the highest enzyme activity, and jasmonic acid (JA) level was higher than in aleurone. In roots, activities of PI4-k, DAG-k, and PA-k were similar, and salicylic acid (SA) showed the highest concentration. In the assays to evaluate the hydrolysis of DGPP (diacylglycerol pyrophosphate) and PA (phosphatidic acid) we observed that PA hydrolysis by LPPs (lipid phosphate phosphatases) was not modified; however, the diacylglycerol pyrophosphate phosphatase (DGPPase) was strikingly higher in coleoptile and root tissues than to aleurone. Relevance of these findings in terms of signaling responses and seedling growth is discussed.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier France-editions Scientifiques Medicales Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Barley  
dc.subject
Lipid Kinases  
dc.subject
Lipid Phosphate Phoshatases  
dc.subject
Phospholipids  
dc.subject
Phytohormones  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Comparative phytohormone pro les, lipid kinase and lipid phosphatase activities in barley aleurone, coleoptile, and root tissues  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-05-10T17:33:11Z  
dc.journal.volume
58  
dc.journal.pagination
83-88  
dc.journal.pais
Francia  
dc.journal.ciudad
París  
dc.description.fil
Fil: Meringer, Maria Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina  
dc.description.fil
Fil: Villasuso, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina  
dc.description.fil
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.description.fil
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.description.fil
Fil: Machado, Estela Edelmira. Universidad Nacional de Río Cuarto; Argentina  
dc.description.fil
Fil: Racagni, Graciela Esther. Universidad Nacional de Río Cuarto; Argentina  
dc.journal.title
Plant Physiology and Biochemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0981942812001556  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2012.06.013