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Artículo

Comparative phytohormone pro les, lipid kinase and lipid phosphatase activities in barley aleurone, coleoptile, and root tissues

Meringer, Maria VeronicaIcon ; Villasuso, Ana LauraIcon ; Pasquaré, Susana Juana; Giusto, Norma MariaIcon ; Machado, Estela Edelmira; Racagni, Graciela Esther
Fecha de publicación: 23/06/2012
Editorial: Elsevier France-editions Scientifiques Medicales Elsevier
Revista: Plant Physiology and Biochemistry
ISSN: 0981-9428
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

We analyzed lipid kinase and lipid phosphatase activities and determined endogenous phytohormone levels by liquid chromatography–tandem mass spectrometry in root and coleoptile tissues following germination of barley (Hordeum vulgare) seeds. The enzymes showing highest activity in aleurone cells were diacylglycerol kinase (DAG-k, EC 2.7.1.107) and phosphatidate kinase (PA-k). The ratio of gibberellins (GAs) to abscisic acid (ABA) was 2-fold higher in aleurone than in coleoptile or root tissues. In coleoptiles, phosphatidylinositol 4-kinase (PI4-k, EC 2.7.1.67) showed the highest enzyme activity, and jasmonic acid (JA) level was higher than in aleurone. In roots, activities of PI4-k, DAG-k, and PA-k were similar, and salicylic acid (SA) showed the highest concentration. In the assays to evaluate the hydrolysis of DGPP (diacylglycerol pyrophosphate) and PA (phosphatidic acid) we observed that PA hydrolysis by LPPs (lipid phosphate phosphatases) was not modified; however, the diacylglycerol pyrophosphate phosphatase (DGPPase) was strikingly higher in coleoptile and root tissues than to aleurone. Relevance of these findings in terms of signaling responses and seedling growth is discussed.
Palabras clave: Barley , Lipid Kinases , Lipid Phosphate Phoshatases , Phospholipids , Phytohormones
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/44876
URL: https://www.sciencedirect.com/science/article/pii/S0981942812001556
DOI: http://dx.doi.org/10.1016/j.plaphy.2012.06.013
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Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Meringer, Maria Veronica; Villasuso, Ana Laura; Pasquaré, Susana Juana; Giusto, Norma Maria; Machado, Estela Edelmira; et al.; Comparative phytohormone pro les, lipid kinase and lipid phosphatase activities in barley aleurone, coleoptile, and root tissues; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 58; 23-6-2012; 83-88
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