Artículo
Biochemical characterization of serine acetyltransferase and cysteine desulfhydrase from Leishmania major
Marciano, Daniela Analia
; Santana, Marianela
; Suárez Mantilla, Brian; Silber, Ariel Mariano; Marino, Cristina Ester
; Nowicki, Cristina
Fecha de publicación:
10/2010
Editorial:
Elsevier Science
Revista:
Molecular and Biochemical Parasitology
ISSN:
0166-6851
e-ISSN:
1872-9428
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cysteine metabolism exhibits atypical features in Leishmania parasites. The nucleotide sequence annotated as LmjF32.2640 encodes a cysteine desulfhydrase, which specifically catalyzes the breakdown of cysteine into pyruvate, NH(3) and H(2)S. Like in other pathogens, this capacity might be associated with regulatory mechanisms to control the intracellular level of cysteine, a highly toxic albeit essential amino acid, in addition to generate pyruvate for energy production. Besides, our results provide the first insight into the biochemical properties of Leishmania major serine acetyltransferase (SAT), which is likely involved in the two routes for de novo synthesis of cysteine in this pathogen. When compared with other members of SAT family, the N-terminal region of L. major homologue is uniquely extended, and seems to be essential for proper protein folding. Furthermore, unlike plant and bacterial enzymes, the carboxy-terminal-C(10) sequence stretch of L. major SAT appears not to be implicated in forming a tight bi-enzyme complex with cysteine synthase.
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Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Marciano, Daniela Analia; Santana, Marianela; Suárez Mantilla, Brian; Silber, Ariel Mariano; Marino, Cristina Ester; et al.; Biochemical characterization of serine acetyltransferase and cysteine desulfhydrase from Leishmania major; Elsevier Science; Molecular and Biochemical Parasitology; 173; 2; 10-2010; 170-174
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