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Artículo

Effects of Organic Solvents on Immobilized Lipase in Pectin Microspheres

Costas, LucianaIcon ; Bosio, Valeria ElizabethIcon ; Pandey, A.; Castro, Guillermo RaulIcon
Fecha de publicación: 12/2008
Editorial: Humana Press
Revista: Applied Biochemistry And Biotechnology
ISSN: 0273-2289
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Lipase from Brevibacillus agri 52 was found stable up to 90% diethylenglycol (DEG), glycerol (GLY), and 1,2 propanediol (1,2 PRO) at 37 °C for 1 h and the stability was reduced only approximately 20% after 12 h incubation, but in 40% dimethylsulfoxide (DMSO), lipase activity was stable only for 1 h. Inhibition of the biocatalysts with dimethylformamide (DMF) was detected at 20% solvent concentration. In water immiscible systems, the stability of lipase in n-hexane, n-tetradecane and n-heptane resembles the water activity, but in the presence of isobutanol, 1-hexanol, and butylbutirate, the stability was significantly reduced. Lipase 52 precipitates in the presence of 50% acetone or ethanol/water mixtures, but enzymatic activity was partially recovered by adding 20% GLY, DEG, 1,2 PRO, or DMSO to the reaction mixture. Furthermore, by increasing DEG in 70% DMF/DEG mixtures, the lipase activity was protected. Encapsulation of lipase in pectin gels cross-linked with calcium ions brings three to four times more enzymatic activity in 70% water miscible organic solvents compared to aqueous systems.
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/43666
DOI: https://dx.doi.org/10.1007/s12010-008-8233-0
URL: https://link.springer.com/article/10.1007%2Fs12010-008-8233-0
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Costas, Luciana; Bosio, Valeria Elizabeth; Pandey, A.; Castro, Guillermo Raul; Effects of Organic Solvents on Immobilized Lipase in Pectin Microspheres; Humana Press; Applied Biochemistry And Biotechnology; 151; 2-3; 12-2008; 578-586
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