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dc.contributor.author
Kato, Hirotomo
dc.contributor.author
Uezato, Hiroshi
dc.contributor.author
Katakura, Ken
dc.contributor.author
Calvopina, Manuel
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Marco, Jorge Diego
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Barroso, Paola Andrea
dc.contributor.author
Gomez, Eduardo
dc.contributor.author
Mimori, Tatsuyuki
dc.contributor.author
Korenaga, Masataka
dc.contributor.author
Iwata, Hiroyuki
dc.contributor.author
Nonaka, Shigeo
dc.contributor.author
Hashiguchi, Yoshihisa
dc.date.available
2018-04-25T14:44:50Z
dc.date.issued
2005-12
dc.identifier.citation
Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge Diego; et al.; Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 72; 1; 12-2005; 87-93
dc.identifier.issn
0002-9637
dc.identifier.uri
http://hdl.handle.net/11336/43443
dc.description.abstract
The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Tropical Medicine and Hygiene
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Tipificación
dc.subject
Leishmania
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Flebótomos
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Pcr
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-03T19:08:45Z
dc.journal.volume
72
dc.journal.number
1
dc.journal.pagination
87-93
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Kato, Hirotomo. Yamaguchi University; Japón
dc.description.fil
Fil: Uezato, Hiroshi. University of the Ryukyus; Japón
dc.description.fil
Fil: Katakura, Ken. Hokkaido University; Japón
dc.description.fil
Fil: Calvopina, Manuel. Kochi University. Kochi Medical School; Japón
dc.description.fil
Fil: Marco, Jorge Diego. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Barroso, Paola Andrea. Kochi University. Kochi Medical School; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Gomez, Eduardo. Universidad Católica de Guayaquil; Ecuador
dc.description.fil
Fil: Mimori, Tatsuyuki. Kumamoto University; Japón
dc.description.fil
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School; Japón
dc.description.fil
Fil: Iwata, Hiroyuki. Yamaguchi University; Japón
dc.description.fil
Fil: Nonaka, Shigeo. University ok the Ryukyus; Japón
dc.description.fil
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; Japón
dc.journal.title
American Journal of Tropical Medicine and Hygiene
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.72.87
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.4269/ajtmh.2005.72.87
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