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Artículo

Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction

Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge DiegoIcon ; Barroso, Paola AndreaIcon ; Gomez, Eduardo; Mimori, Tatsuyuki; Korenaga, Masataka; Iwata, Hiroyuki; Nonaka, Shigeo; Hashiguchi, Yoshihisa
Fecha de publicación: 12/2005
Editorial: American Society of Tropical Medicine and Hygiene
Revista: American Journal of Tropical Medicine and Hygiene
ISSN: 0002-9637
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.
Palabras clave: Tipificación , Leishmania , Flebótomos , Pcr
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/43443
URL: http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.72.87
DOI: https://dx.doi.org/10.4269/ajtmh.2005.72.87
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Articulos(IPE)
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Citación
Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Calvopina, Manuel; Marco, Jorge Diego; et al.; Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 72; 1; 12-2005; 87-93
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