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Artículo

Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876

Cavello, Ivana AlejandraIcon ; Cavalitto, Sebastian FernandoIcon ; Hours, Roque AlbertoIcon
Fecha de publicación: 06/2013
Editorial: Korean Society Microbiology
Revista: Journal of Microbiology and Biotechnology
ISSN: 1017-7825
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología Medioambiental

Resumen

Enzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films.
Palabras clave: Silver Recovery , Serine Proteases , X-Ray Films , Thermostability
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/4325
URL: https://www.jmb.or.kr/journal/view.html?volume=23&number=8&spage=1133
Colecciones
Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Citación
Cavello, Ivana Alejandra; Cavalitto, Sebastian Fernando; Hours, Roque Alberto; Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876; Korean Society Microbiology; Journal of Microbiology and Biotechnology; 23; 8; 6-2013; 1133-1139
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