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dc.contributor.author Sobrero, Patricio Martín
dc.contributor.author Muzlera, Andrés
dc.contributor.author Frescura, Julieta Magali
dc.contributor.author Jofré, Edgardo
dc.contributor.author Valverde, Claudio Fabián
dc.date.available 2018-04-20T14:44:30Z
dc.date.issued 2017-10
dc.identifier.citation Sobrero, Patricio Martín; Muzlera, Andrés; Frescura, Julieta Magali; Jofré, Edgardo; Valverde, Claudio Fabián; A matter of hierarchy: Activation of orfamide production by the posttranscriptional Gac-Rsm cascade of Pseudomonas protegens CHA0 through expression upregulation of the two dedicated transcriptional regulators.; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 9; 5; 10-2017; 599-611
dc.identifier.issn 1462-2912
dc.identifier.uri http://hdl.handle.net/11336/42818
dc.description.abstract In this work, we surveyed the genome of P. protegens CHA0 in order to identify novel mRNAs possibly under the control of the Gac-Rsm cascade that might, for their part, serve to elucidate as-yet-unknown functions involved in the biocontrol of plant pathogens and/or in cellular processes required for fitness in natural environments. In view of the experimental evidence from former studies on the Gac-Rsm cascade, we developed a computational screen supported by a combination of sequence, structural, and evolutionary constraints that led to a dataset of 43 potential novel mRNA targets. We then confirmed several mRNA targets experimentally and next focused on two of the respective genes that are physically linked to the orfamide biosynthetic gene cluster and whose predicted open-reading frames resembled cognate LuxR-type transcriptional regulators of cyclic lipopeptide clusters in related pseudomonads. In this report, we demonstrate that in strain CHA0, orfamide production is stringently dependent on a functional Gac-Rsm cascade and that both mRNAs encoding transcriptional regulatory proteins are under direct translational control of the RsmA/E proteins. Our results have thus revealed a hierarchical control over the expression of orfamide biosynthetic genes with the final transcriptional control subordinated to the global Gac-Rsm posttranscriptional regulatory system.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject PSEUDOMONAS PROTEGENS
dc.subject GAC-RSM
dc.subject POSTTRANSCRIPTIONAL CONTROL
dc.subject ORFAMIDE
dc.subject TRANSCRIPTIONAL REGULATOR
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title A matter of hierarchy: Activation of orfamide production by the posttranscriptional Gac-Rsm cascade of Pseudomonas protegens CHA0 through expression upregulation of the two dedicated transcriptional regulators.
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-06T13:53:03Z
dc.journal.volume 9
dc.journal.number 5
dc.journal.pagination 599-611
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Sobrero, Patricio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
dc.description.fil Fil: Muzlera, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
dc.description.fil Fil: Frescura, Julieta Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
dc.description.fil Fil: Jofré, Edgardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina
dc.description.fil Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
dc.journal.title Environmental Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/1758-2229.12566
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/1758-2229.12566
dc.conicet.fuente Elsevier


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)