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Artículo

A matter of hierarchy: Activation of orfamide production by the posttranscriptional Gac-Rsm cascade of Pseudomonas protegens CHA0 through expression upregulation of the two dedicated transcriptional regulators.

Sobrero, Patricio MartínIcon ; Muzlera, AndrésIcon ; Frescura, Julieta Magali; Jofré, EdgardoIcon ; Valverde, Claudio FabiánIcon
Fecha de publicación: 10/2017
Editorial: Wiley Blackwell Publishing, Inc
Revista: Environmental Microbiology
ISSN: 1462-2912
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

In this work, we surveyed the genome of P. protegens CHA0 in order to identify novel mRNAs possibly under the control of the Gac-Rsm cascade that might, for their part, serve to elucidate as-yet-unknown functions involved in the biocontrol of plant pathogens and/or in cellular processes required for fitness in natural environments. In view of the experimental evidence from former studies on the Gac-Rsm cascade, we developed a computational screen supported by a combination of sequence, structural, and evolutionary constraints that led to a dataset of 43 potential novel mRNA targets. We then confirmed several mRNA targets experimentally and next focused on two of the respective genes that are physically linked to the orfamide biosynthetic gene cluster and whose predicted open-reading frames resembled cognate LuxR-type transcriptional regulators of cyclic lipopeptide clusters in related pseudomonads. In this report, we demonstrate that in strain CHA0, orfamide production is stringently dependent on a functional Gac-Rsm cascade and that both mRNAs encoding transcriptional regulatory proteins are under direct translational control of the RsmA/E proteins. Our results have thus revealed a hierarchical control over the expression of orfamide biosynthetic genes with the final transcriptional control subordinated to the global Gac-Rsm posttranscriptional regulatory system.
Palabras clave: Pseudomonas Protegens , Gac-Rsm , Posttranscriptional Control , Orfamide , Transcriptional Regulator
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/42818
DOI: http://dx.doi.org/10.1111/1758-2229.12566
URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/1758-2229.12566
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Articulos de SEDE CENTRAL
Citación
Sobrero, Patricio Martín; Muzlera, Andrés; Frescura, Julieta Magali; Jofré, Edgardo; Valverde, Claudio Fabián; A matter of hierarchy: Activation of orfamide production by the posttranscriptional Gac-Rsm cascade of Pseudomonas protegens CHA0 through expression upregulation of the two dedicated transcriptional regulators.; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 9; 5; 10-2017; 599-611
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