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dc.contributor.author
Panei, Carlos Javier
dc.contributor.author
Takeshima, Shin-nosuke
dc.contributor.author
Omori, Takashi
dc.contributor.author
Nunoya, Tetsuo
dc.contributor.author
Davis, William C.
dc.contributor.author
Ishizaki, Hiroshi
dc.contributor.author
Matoba, Kazuhiro
dc.contributor.author
Aida, Yoko
dc.date.available
2018-04-10T20:24:42Z
dc.date.issued
2013-03
dc.identifier.citation
Panei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; et al.; Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR; BioMed Central; BMC Veterinary Research; 9; 95; 3-2013; 1-8
dc.identifier.issn
1746-6148
dc.identifier.uri
http://hdl.handle.net/11336/41613
dc.description.abstract
Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bovine Leukemia Virus (Blv)
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Proviral Load
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Blv-Cocomo-Qpcr
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Cd5+Igm+ Cell
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Flow Cytometry
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Cell Sorting
dc.subject.classification
Zoología, Ornitología, Entomología, Etología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Producción Animal y Lechería
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Producción Animal y Lechería
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CIENCIAS AGRÍCOLAS
dc.title
Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-10T19:02:58Z
dc.journal.volume
9
dc.journal.number
95
dc.journal.pagination
1-8
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Panei, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Discovery Riken Institute; Japón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Takeshima, Shin-nosuke. Discovery Riken Institute; Japón
dc.description.fil
Fil: Omori, Takashi. Nippon Institute For Biological Sciences; Japón
dc.description.fil
Fil: Nunoya, Tetsuo. Nippon Institute For Biological Sciences; Japón
dc.description.fil
Fil: Davis, William C.. Washington State University; Estados Unidos
dc.description.fil
Fil: Ishizaki, Hiroshi. Naro Institute Of Livestock And Grassland Sciences; Japón
dc.description.fil
Fil: Matoba, Kazuhiro. Naro Institute Of Livestock And Grassland Sciences; Japón
dc.description.fil
Fil: Aida, Yoko. Discovery Riken Institute; Japón
dc.journal.title
BMC Veterinary Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-9-95
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1746-6148-9-95
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