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Artículo

Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR

Panei, Carlos JavierIcon ; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; Ishizaki, Hiroshi; Matoba, Kazuhiro; Aida, Yoko
Fecha de publicación: 03/2013
Editorial: BioMed Central
Revista: BMC Veterinary Research
ISSN: 1746-6148
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Zoología, Ornitología, Entomología, Etología; Producción Animal y Lechería

Resumen

Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR
Palabras clave: Bovine Leukemia Virus (Blv) , Proviral Load , Blv-Cocomo-Qpcr , Cd5+Igm+ Cell , Flow Cytometry , Cell Sorting
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/41613
URL: https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-9-95
DOI: http://dx.doi.org/10.1186/1746-6148-9-95
Colecciones
Articulos(CEPAVE)
Articulos de CENTRO DE EST.PARASITOL.Y DE VECTORES (I)
Citación
Panei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; et al.; Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR; BioMed Central; BMC Veterinary Research; 9; 95; 3-2013; 1-8
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