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dc.contributor.author Policastro, Lucia Laura
dc.contributor.author Molinari, Beatriz Lucia
dc.contributor.author Larcher, Fernando
dc.contributor.author Blanco, Patricia
dc.contributor.author Podhajcer, Osvaldo Luis
dc.contributor.author Costa, Cristina Susana
dc.contributor.author Rojas, Paola Andrea
dc.contributor.author Duran, Hebe Alicia
dc.date.available 2018-04-10T17:14:16Z
dc.date.issued 2004-02
dc.identifier.citation Policastro, Lucia Laura; Molinari, Beatriz Lucia; Larcher, Fernando; Blanco, Patricia; Podhajcer, Osvaldo Luis; et al.; Imbalance of antioxidant enzymes in tumor cells and inhibition of proliferation and malignant features by scavenging hydrogen peroxide; Wiley-liss, Div John Wiley & Sons Inc; Molecular Carcinogenesis; 39; 2; 2-2004; 103-113
dc.identifier.issn 0899-1987
dc.identifier.uri http://hdl.handle.net/11336/41523
dc.description.abstract The aim of this study was to evaluate the endogenous alterations of the antioxidant enzymes in tumor cells and to specifically compensate the resulting changes in the levels of reactive oxygen species (ROS) to control the malignant growth. We determined and compared the activities of antioxidant enzymes and the levels of superoxide anion (O2*-) and hydrogen peroxide (H2O2) in tumor cell lines with different degrees of malignancy, paired with regard to their origin (PB/CH72T4, PDV/PDVC57, and HBL-100/MCF-7). An increase in superoxide dismutase activity and a decrease in the activities of H2O2-detoxifying enzymes, as a function of malignancy, coupled with a rise in H2O2 and a decrease in O2*- were demonstrated. Treatment of cells with exogenous catalase showed a dose-dependent inhibition of proliferation. This inhibition was also demonstrated in several cell lines of different tissue origin and species, suggesting a general role of H2O2 in cell proliferation. Moreover, stable expression of human catalase in MCF-7 cells inhibited proliferation and also reverted malignant features. We conclude that H2O2 played a crucial and general role in the regulation of proliferation and that an endogenous imbalance in antioxidant enzymes could be a relevant event in the carcinogenesis process.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley-liss, Div John Wiley & Sons Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CARCINOGENESIS
dc.subject ROS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Imbalance of antioxidant enzymes in tumor cells and inhibition of proliferation and malignant features by scavenging hydrogen peroxide
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-10T13:51:09Z
dc.journal.volume 39
dc.journal.number 2
dc.journal.pagination 103-113
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.description.fil Fil: Policastro, Lucia Laura. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Molinari, Beatriz Lucia. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Larcher, Fernando. Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas; España
dc.description.fil Fil: Blanco, Patricia. Fundación Instituto Leloir; Argentina
dc.description.fil Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica; Argentina
dc.description.fil Fil: Rojas, Paola Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Duran, Hebe Alicia. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Molecular Carcinogenesis
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/mc.20001
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/mc.20001
dc.conicet.fuente unificacion


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)