Artículo
Imbalance of antioxidant enzymes in tumor cells and inhibition of proliferation and malignant features by scavenging hydrogen peroxide
Policastro, Lucia Laura
; Molinari, Beatriz Lucia
; Larcher, Fernando; Blanco, Patricia; Podhajcer, Osvaldo Luis
; Costa, Cristina Susana; Rojas, Paola Andrea
; Duran, Hebe Alicia
Fecha de publicación:
02/2004
Editorial:
Wiley-liss, Div John Wiley & Sons Inc
Revista:
Molecular Carcinogenesis
ISSN:
0899-1987
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this study was to evaluate the endogenous alterations of the antioxidant enzymes in tumor cells and to specifically compensate the resulting changes in the levels of reactive oxygen species (ROS) to control the malignant growth. We determined and compared the activities of antioxidant enzymes and the levels of superoxide anion (O2*-) and hydrogen peroxide (H2O2) in tumor cell lines with different degrees of malignancy, paired with regard to their origin (PB/CH72T4, PDV/PDVC57, and HBL-100/MCF-7). An increase in superoxide dismutase activity and a decrease in the activities of H2O2-detoxifying enzymes, as a function of malignancy, coupled with a rise in H2O2 and a decrease in O2*- were demonstrated. Treatment of cells with exogenous catalase showed a dose-dependent inhibition of proliferation. This inhibition was also demonstrated in several cell lines of different tissue origin and species, suggesting a general role of H2O2 in cell proliferation. Moreover, stable expression of human catalase in MCF-7 cells inhibited proliferation and also reverted malignant features. We conclude that H2O2 played a crucial and general role in the regulation of proliferation and that an endogenous imbalance in antioxidant enzymes could be a relevant event in the carcinogenesis process.
Palabras clave:
Carcinogenesis
,
Ros
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Policastro, Lucia Laura; Molinari, Beatriz Lucia; Larcher, Fernando; Blanco, Patricia; Podhajcer, Osvaldo Luis; et al.; Imbalance of antioxidant enzymes in tumor cells and inhibition of proliferation and malignant features by scavenging hydrogen peroxide; Wiley-liss, Div John Wiley & Sons Inc; Molecular Carcinogenesis; 39; 2; 2-2004; 103-113
Compartir
Altmétricas