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Artículo

Recombinant invasive Lactococcus lactis carrying a DNA vaccine coding the Ag85A antigen increases INF-γ, IL-6, and TNF-α cytokines after intranasal immunization

Mancha Agresti, Pamela; Castro, Camila Prosperi de; Santos, Janete S. C. dos; Araujo, Maíra A.; Pereira, Vanessa B.; Leblanc, Jean Guy JosephIcon ; Leclercq, Sophie Y.; Azevedo, Vasco
Fecha de publicación: 07/2017
Editorial: Frontiers Research Foundation
Revista: Frontiers in Microbiology
ISSN: 1664-302X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Tuberculosis (TB) remains a major threat throughout the world and in 2015 it caused the death of 1.4 million people. The Bacillus Calmette-Guérin is the only existing vaccine against this ancient disease; however, it does not provide complete protection in adults. New vaccines against TB are eminently a global priority. The use of bacteria as vehicles for delivery of vaccine plasmids is a promising vaccination strategy. In this study, we evaluated the use of, an engineered invasive Lactococcus lactis (expressing Fibronectin-Binding Protein A from Staphylococcus aureus) for the delivery of DNA plasmid to host cells, especially to the mucosal site as a new DNA vaccine against tuberculosis. One of the major antigens documented that offers protective responses against Mycobacterium tuberculosis is the Ag85A. L. lactis FnBPA+ (pValac:Ag85A) which was obtained and used for intranasal immunization of C57BL/6 mice and the immune response profile was evaluated. In this study we observed that this strain was able to produce significant increases in the amount of pro-inflammatory cytokines (IFN-γ, TNF-α, and IL-6) in the stimulated spleen cell supernatants, showing a systemic T helper 1 (Th1) cell response. Antibody production (IgG and sIgA anti-Ag85A) was also significantly increased in bronchoalveolar lavage, as well as in the serum of mice. In summary, these findings open new perspectives in the area of mucosal DNA vaccine, against specific pathogens using a Lactic Acid Bacteria such as L. lactis.
Palabras clave: Ag85a , Lactic Acid Bacteria , Lactococcus Lactis , Mucosal Vaccine , Tuberculosis
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/40080
DOI: http://dx.doi.org/10.3389/fmicb.2017.01263
URL: https://www.frontiersin.org/articles/10.3389/fmicb.2017.01263/full
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Mancha Agresti, Pamela; Castro, Camila Prosperi de; Santos, Janete S. C. dos ; Araujo, Maíra A.; Pereira, Vanessa B.; et al.; Recombinant invasive Lactococcus lactis carrying a DNA vaccine coding the Ag85A antigen increases INF-γ, IL-6, and TNF-α cytokines after intranasal immunization; Frontiers Research Foundation; Frontiers in Microbiology; 8; JUL; 7-2017; 1-12; 1263
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