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dc.contributor.author
Pisa, José Horacio
dc.contributor.author
Manfredi, Adriana Paola
dc.contributor.author
Perotti, Nora Ines
dc.contributor.author
Romero, Héctor G.
dc.contributor.author
Breccia, Javier Dario
dc.contributor.author
Martinez, Maria Alejandra
dc.date.available
2018-03-21T20:02:16Z
dc.date.issued
2017-11
dc.identifier.citation
Pisa, José Horacio; Manfredi, Adriana Paola; Perotti, Nora Ines; Romero, Héctor G.; Breccia, Javier Dario; et al.; Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92; Karger; Journal of Molecular Microbiology and Biotechnology; 27; 5; 11-2017; 277-288
dc.identifier.issn
1464-1801
dc.identifier.uri
http://hdl.handle.net/11336/39560
dc.description.abstract
Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cohnella
dc.subject
Hemicellulose Degradation
dc.subject
Paenibacillaceae
dc.subject
Sugarcane Bagasse
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-21T15:54:37Z
dc.journal.volume
27
dc.journal.number
5
dc.journal.pagination
277-288
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Manfredi, Adriana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Romero, Héctor G.. Universidad de la República. Facultad de Ciencias; Uruguay
dc.description.fil
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
dc.description.fil
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Journal of Molecular Microbiology and Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/480541
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000480541


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