Artículo
LIN7A is a major determinant of cell-polarity defects in breast carcinomas
Gruel, Nadège; Fuhrmann, Laetitia; Lodillinsky, Catalina
; Benhamo, Vanessa; Mariani, Odette; Cédenot, Aurélie; Arnould, Laurent; Macgrogan, Gaëtan; Sastre-Garau, Xavier; Chavrier, Philippe; Delattre, Olivier; Vincent Salomon, Anne
Fecha de publicación:
02/2016
Editorial:
BioMed Central
Revista:
Breast Cancer Research
ISSN:
1465-5411
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Polarity defects are a hallmark of most carcinomas. Cells from invasive micropapillary carcinomas (IMPCs) of the breast are characterized by a striking cell polarity inversion and represent an interesting model for the analysis of polarity abnormalities. Methods: In-depth investigation of polarity proteins in 24 IMPCs and a gene expression profiling, comparing IMPC (n = 73) with invasive carcinomas of no special type (ICNST) (n = 51) have been performed. Results: IMPCs showed a profound disorganization of the investigated polarity proteins and revealed major abnormalities in their subcellular localization. Gene expression profiling experiments highlighted a number of deregulated genes in the IMPCs that have a role in apico-basal polarity, adhesion and migration. LIN7A, a Crumbs-complex polarity gene, was one of the most differentially over-expressed genes in the IMPCs. Upon LIN7A over-expression, we observed hyperproliferation, invasion and a complete absence of lumen formation, revealing strong polarity defects. Conclusion: This study therefore shows that LIN7A has a crucial role in the polarity abnormalities associated with breast carcinogenesis.
Palabras clave:
Breast Cancer
,
Cell Polarity
,
Lin7a
,
Micropapillary Carcinomas
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Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Gruel, Nadège; Fuhrmann, Laetitia; Lodillinsky, Catalina; Benhamo, Vanessa; Mariani, Odette; et al.; LIN7A is a major determinant of cell-polarity defects in breast carcinomas; BioMed Central; Breast Cancer Research; 18; 1; 2-2016; 1-10
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