Artículo
Natural occurring epialleles determine vitamin E accumulation in tomato fruits
Quadrana, Leandro Daniel
; Almeida de Souza, Juliana Beatriz; Asis, Ramón
; Duffy, Tomás
; Dominguez, Pia Guadalupe
; Bermudez Salazar, Luisa Fernanda
; Conti, Gabriela
; Silva, Junia V. Corrêa da; Peralta, Iris Edith
; Colot, Vincent; Asurmendi, Sebastian
; Fernie, Alisdair R.; Rossi, Magdalena; Carrari, Fernando Oscar
Fecha de publicación:
06/2014
Editorial:
Nature
Revista:
Nature Communications
ISSN:
2041-1723
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Vitamin E (VTE) content is a low heritability nutritional trait for which the genetic determinants are poorly understood. Here, we focus on a previously detected major tomato VTE quantitative trait loci (QTL; mQTL 9-2-6) and identify the causal gene as one encoding a 2-methyl-6-phytylquinol methyltransferase (namely VTE3(1)) that catalyses one of the final steps in the biosynthesis of γ 3- and α -tocopherols, which are the main forms of VTE. By reverse genetic approaches, expression analyses, siRNA profiling and DNA methylation assays, we demonstrate that mQTL9-2-6 is an expression QTL associated with differential methylation of a SINE retrotransposon located in the promoter region of VTE3(1). Promoter DNA methylation can be spontaneously reverted leading to different epialleles affecting VTE3(1) expression and VTE content in fruits. These findings indicate therefore that naturally occurring epialleles are responsible for regulation of a nutritionally important metabolic QTL and provide direct evidence of a role for epigenetics in the determination of agronomic traits.
Palabras clave:
Tomato
,
Fruits
,
Epigenetics
,
Vitamine C
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos(IADIZA)
Articulos de INST. ARG DE INVEST. DE LAS ZONAS ARIDAS
Articulos de INST. ARG DE INVEST. DE LAS ZONAS ARIDAS
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Quadrana, Leandro Daniel; Almeida de Souza, Juliana Beatriz; Asis, Ramón; Duffy, Tomás; Dominguez, Pia Guadalupe; et al.; Natural occurring epialleles determine vitamin E accumulation in tomato fruits; Nature; Nature Communications; 5; 4027; 6-2014; 3027-3057
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