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dc.contributor.author
Zhang, D.
dc.contributor.author
Choi, D. W.
dc.contributor.author
Wanamaker, S.
dc.contributor.author
Fenton, R. D.
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Chin, A.
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Malatrasi, M.
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Turuspekov, Y.
dc.contributor.author
Walia, H.
dc.contributor.author
Akhunov, E.D.
dc.contributor.author
Kianian, P.
dc.contributor.author
Otto, C.
dc.contributor.author
Simons, K.
dc.contributor.author
Deal, K.R.
dc.contributor.author
Echenique, Carmen Viviana
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Stamova, B.
dc.contributor.author
Ross, K.
dc.contributor.author
Butler, G. E.
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Strader, L.
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Verhey, S.D.
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Johnson, R.
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Altenbach, S.
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Kothari, K.
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Shah, M. M.
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Laudencia Chingcuanco, D.
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Han, P.
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Miller, R. E.
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Crossman, C. C.
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Chao, S.
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Lazo, G. R.
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Klueva, N.
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Gustafson, J. P.
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Kianian, S. F.
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Dubcovsky, J.
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Walker Simmons, M. K.
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Gill, K. S.
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Dvořák, J.
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Anderson, O. D.
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Sorrells, M. E.
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McGuire, P. E.
dc.contributor.author
Qualset, C. O.
dc.contributor.author
Nguyen, H. T.
dc.contributor.author
Close, T. J.
dc.date.available
2018-01-23T13:46:06Z
dc.date.issued
2004-10
dc.identifier.citation
Zhang, D.; Choi, D. W.; Wanamaker, S.; Fenton, R. D.; Chin, A.; et al.; Construction and Evaluation of cDNA Libraries for Large-Scale Expressed Sequence Tag Sequencing in Wheat (Triticum aestivum L.); Genetics Soc Am; Genetics; 168; 2; 10-2004; 595-608
dc.identifier.issn
0016-6731
dc.identifier.uri
http://hdl.handle.net/11336/34244
dc.description.abstract
A total of 37 original cDNA libraries and 9 derivative libraries enriched for rare sequences were produced from Chinese Spring wheat (Triticum aestivum L.), five other hexaploid wheat genotypes (Cheyenne, Brevor, TAM W101, BH1146, Butte 86), tetraploid durum wheat (T. turgidum L.), diploid wheat (T. monococcum L.), and two other diploid members of the grass tribe Triticeae (Aegilops speltoides Tausch and Secale cereale L.). The emphasis in the choice of plant materials for library construction was reproductive development subjected to environmental factors that ultimately affect grain quality and yield, but roots and other tissues were also included. Partial cDNA expressed sequence tags (ESTs) were examined by various measures to assess the quality of these libraries. All ESTs were processed to remove cloning system sequences and contaminants and then assembled using CAP3. Following these processing steps, this assembly yielded 101,107 sequences derived from 89,043 clones, which defined 16,740 contigs and 33,213 singletons, a total of 49,953 “unigenes.” Analysis of the distribution of these unigenes among the libraries led to the conclusion that the enrichment methods were effective in reducing the most abundant unigenes and to the observation that the most diverse libraries were from tissues exposed to environmental stresses including heat, drought, salinity, or low temperature.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Genetics Soc Am
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Triticum Aestivum
dc.subject
Cdna
dc.subject
Libraries
dc.subject
Ests
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Construction and Evaluation of cDNA Libraries for Large-Scale Expressed Sequence Tag Sequencing in Wheat (Triticum aestivum L.)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-16T14:58:29Z
dc.journal.volume
168
dc.journal.number
2
dc.journal.pagination
595-608
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Zhang, D.. University of Texas; Estados Unidos
dc.description.fil
Fil: Choi, D. W.. University of California; Estados Unidos
dc.description.fil
Fil: Wanamaker, S.. University of California; Estados Unidos
dc.description.fil
Fil: Fenton, R. D.. University of California; Estados Unidos
dc.description.fil
Fil: Chin, A.. University of California; Estados Unidos
dc.description.fil
Fil: Malatrasi, M.. University of California; Estados Unidos
dc.description.fil
Fil: Turuspekov, Y.. University of California; Estados Unidos
dc.description.fil
Fil: Walia, H.. University of California; Estados Unidos
dc.description.fil
Fil: Akhunov, E.D.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Kianian, P.. North Dakota State University ; Estados Unidos
dc.description.fil
Fil: Otto, C.. North Dakota State University ; Estados Unidos
dc.description.fil
Fil: Simons, K.. North Dakota State University ; Estados Unidos
dc.description.fil
Fil: Deal, K.R.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Stamova, B.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Ross, K.. University of Missouri; Estados Unidos
dc.description.fil
Fil: Butler, G. E.. University of Arizona; Estados Unidos
dc.description.fil
Fil: Strader, L.. Washington State University; Estados Unidos
dc.description.fil
Fil: Verhey, S.D.. Washington State University; Estados Unidos
dc.description.fil
Fil: Johnson, R.. Washington State University; Estados Unidos
dc.description.fil
Fil: Altenbach, S.. United States Departamento of Agriculture; Albania
dc.description.fil
Fil: Kothari, K.. United States Department of Agriculture; Estados Unidos
dc.description.fil
Fil: Shah, M. M.. North Dakota State University ; Estados Unidos
dc.description.fil
Fil: Laudencia Chingcuanco, D.. United States Department of Agriculture; Estados Unidos
dc.description.fil
Fil: Han, P.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Miller, R. E.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Crossman, C. C.. United States Department of Agriculture; Estados Unidos
dc.description.fil
Fil: Chao, S.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Lazo, G. R.. United States Department of Agriculture; Estados Unidos
dc.description.fil
Fil: Klueva, N.. University of Texas; Estados Unidos
dc.description.fil
Fil: Gustafson, J. P.. University of Missouri; Estados Unidos
dc.description.fil
Fil: Kianian, S. F.. North Dakota State University ; Estados Unidos
dc.description.fil
Fil: Dubcovsky, J.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Walker Simmons, M. K.. Washington State University; Estados Unidos
dc.description.fil
Fil: Gill, K. S.. Washington State University; Estados Unidos
dc.description.fil
Fil: Dvořák, J.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Anderson, O. D.. United States Department of Agriculture; Estados Unidos
dc.description.fil
Fil: Sorrells, M. E.. Cornell University; Estados Unidos
dc.description.fil
Fil: McGuire, P. E.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Qualset, C. O.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Nguyen, H. T.. University of Missouri; Estados Unidos
dc.description.fil
Fil: Close, T. J.. University of California; Estados Unidos
dc.journal.title
Genetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.genetics.org/content/168/2/595
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1534/genetics.104.034785


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