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Artículo

Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate

Ghiringhelli, Pablo DanielIcon ; Hours, Roque AlbertoIcon ; Chesini, MarianaIcon ; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; Rojas, Natalia LorenaIcon ; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastian FernandoIcon
Fecha de publicación: 05/2013
Editorial: Pontificia Universidad Catolica de Valparaíso
Revista: Electronic Journal Of Biotechnology
ISSN: 0717-3458
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación

Resumen

Inulinases have been extracted and characterized from inulin-storing tissues,however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
Palabras clave: Acidophilic Enzymes , Batch Cultures , Inulin Degradation , Submerged Cultures
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Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/3385
DOI: http://dx.doi.org/ 10.2225/vol16-issue3-fulltext-13
URL: http://ref.scielo.org/4h9fbx
DOI: http://dx.doi.org/10.2225/vol16-issue3-fulltext-13
URL: http://dx.doi.org/ www.ejbiotechnology.info/index.php/ejbiotechnology/rt/printerFriendly/v16n3-13/1
Colecciones
Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Ghiringhelli, Pablo Daniel; Hours, Roque Alberto; Chesini, Mariana; Neila, Lorena Paola; Fratebianchi de la Parra, Dante; et al.; Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-13
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