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dc.contributor.author
Cappa, Valeria Alejandra
dc.contributor.author
Rivero, Cintia Wanda
dc.contributor.author
Britos, Claudia Noelia
dc.contributor.author
Martínez, Luis M.
dc.contributor.author
Lozano, Mario Enrique
dc.contributor.author
Trelles, Jorge Abel
dc.date.available
2018-01-11T19:51:15Z
dc.date.issued
2014-04
dc.identifier.citation
Cappa, Valeria Alejandra; Trelles, Jorge Abel; Rivero, Cintia Wanda; Lozano, Mario Enrique; Martínez, Luis M.; Britos, Claudia Noelia; et al.; An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate; Elsevier; Process Biochemistry; 49; 7; 4-2014; 1169-1175
dc.identifier.issn
1359-5113
dc.identifier.uri
http://hdl.handle.net/11336/33028
dc.description.abstract
An efficient and green immobilized biocatalyst is herein reported to obtain 5-fluorouracil-2′-deoxyriboside (5FUradRib), an antimetabolite known as Floxuridine, used in gastrointestinal cancer treatment. Alginate is a natural polysaccharide used in the pharmaceutical industry due to its physicochemical properties, biocompatibility and non-toxicity. Multivalent cations, exposure time and cross-linking solution concentration were optimized, being Sr2+, 2 h and 0.2 M the best immobilization conditions. Furthermore, compression strength, swelling ratio and fracture frequency were evaluated, improving the mechanical stability of the biocatalyst favoring a future scale-up. On the other hand, the reaction parameters for 5FUradRib biosynthesis were optimized in order to obtain an immobilized biocatalyst with enhanced activity. Thus, Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate showed yields of 96% at short reaction times. The obtained biocatalyst was stable for more than 25 days in storage conditions (4 °C) and could be reused at least 10 times without loss of its activity. Additionally, Sr-alginate biocatalyst stability was evaluated in different organic solvents to obtain hydrophobic compounds such as 5-bromouracil-2′-deoxyriboside (5BrUradRib), an effective radiosensitizing agent used in anti-cancer therapy, being hexane the best co-solvent. Finally, a smooth, cheap and environmentally friendly method to obtain anti-cancer drugs was developed in this study.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lactic-Acid-Bacteria
dc.subject
Halogenated-2′-Deoxyribosides
dc.subject
Microorganism-Stabilization
dc.subject
Anti-Tumoral-Compounds
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Co-Solvents
dc.subject.classification
Otras Biotecnologías de la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-11T13:48:27Z
dc.journal.volume
49
dc.journal.number
7
dc.journal.pagination
1169-1175
dc.journal.pais
Países Bajos
dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Cappa, Valeria Alejandra. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina
dc.description.fil
Fil: Britos, Claudia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina
dc.description.fil
Fil: Martínez, Luis M.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina
dc.description.fil
Fil: Lozano, Mario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina
dc.description.fil
Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Process Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.procbio.2014.03.029
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511314002244


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