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Artículo

An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate

Cappa, Valeria AlejandraIcon ; Rivero, Cintia WandaIcon ; Britos, Claudia NoeliaIcon ; Martínez, Luis M.; Lozano, Mario EnriqueIcon ; Trelles, Jorge AbelIcon
Fecha de publicación: 04/2014
Editorial: Elsevier
Revista: Process Biochemistry
ISSN: 1359-5113
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnologías de la Salud

Resumen

An efficient and green immobilized biocatalyst is herein reported to obtain 5-fluorouracil-2′-deoxyriboside (5FUradRib), an antimetabolite known as Floxuridine, used in gastrointestinal cancer treatment. Alginate is a natural polysaccharide used in the pharmaceutical industry due to its physicochemical properties, biocompatibility and non-toxicity. Multivalent cations, exposure time and cross-linking solution concentration were optimized, being Sr2+, 2 h and 0.2 M the best immobilization conditions. Furthermore, compression strength, swelling ratio and fracture frequency were evaluated, improving the mechanical stability of the biocatalyst favoring a future scale-up. On the other hand, the reaction parameters for 5FUradRib biosynthesis were optimized in order to obtain an immobilized biocatalyst with enhanced activity. Thus, Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate showed yields of 96% at short reaction times. The obtained biocatalyst was stable for more than 25 days in storage conditions (4 °C) and could be reused at least 10 times without loss of its activity. Additionally, Sr-alginate biocatalyst stability was evaluated in different organic solvents to obtain hydrophobic compounds such as 5-bromouracil-2′-deoxyriboside (5BrUradRib), an effective radiosensitizing agent used in anti-cancer therapy, being hexane the best co-solvent. Finally, a smooth, cheap and environmentally friendly method to obtain anti-cancer drugs was developed in this study.
Palabras clave: Lactic-Acid-Bacteria , Halogenated-2′-Deoxyribosides , Microorganism-Stabilization , Anti-Tumoral-Compounds , Co-Solvents
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/33028
DOI: http://dx.doi.org/10.1016/j.procbio.2014.03.029
URL: https://www.sciencedirect.com/science/article/pii/S1359511314002244
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Cappa, Valeria Alejandra; Trelles, Jorge Abel; Rivero, Cintia Wanda; Lozano, Mario Enrique; Martínez, Luis M.; Britos, Claudia Noelia; et al.; An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate; Elsevier; Process Biochemistry; 49; 7; 4-2014; 1169-1175
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