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dc.contributor.author
Lepanto, Paola  
dc.contributor.author
Lecumberry, Federico  
dc.contributor.author
Rossello, Jéssica  
dc.contributor.author
Kierbel, Arlinet Verónica  
dc.date.available
2018-01-04T19:17:34Z  
dc.date.issued
2014-02  
dc.identifier.citation
Kierbel, Arlinet Verónica; Rossello, Jéssica; Lecumberry, Federico; Lepanto, Paola; A confocal microscopy image analysis method to measure adhesion and internalization of Pseudomonas aeruginosa multicellular structures into epithelial cells; Academic Press Ltd-elsevier Science Ltd; Molecular And Cellular Probes; 28; 1; 2-2014; 1-5  
dc.identifier.issn
0890-8508  
dc.identifier.uri
http://hdl.handle.net/11336/32351  
dc.description.abstract
Formation of multicellular structures such as biofilms is an important feature in the physiopathology of many disease-causing bacteria. We recently reported that Pseudomonas aeruginosa adheres to epithelial cells rapidly forming early biofilm-like aggregates, which can then be internalized into cells. Conventional methods to measure adhesion/internalization, such as dilution plating for total cell-associated or antibiotic protected bacteria, do not distinguish between single and aggregated bacteria. We report a procedure that combining double bacteria labeling, confocal microscopy and image analysis allows identification and quantification of the number of adhered and internalized bacteria distinguishing between single and aggregated bacterial cells. A plugin for Fiji to automatically perform these procedures has been generated.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Academic Press Ltd-elsevier Science Ltd  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Pseudomonas Aeruginosa  
dc.subject
Adhesion  
dc.subject
Internalization  
dc.subject
Multicellular Structures  
dc.subject
Image Analysis  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
A confocal microscopy image analysis method to measure adhesion and internalization of Pseudomonas aeruginosa multicellular structures into epithelial cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-01-03T19:03:51Z  
dc.journal.volume
28  
dc.journal.number
1  
dc.journal.pagination
1-5  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
New York  
dc.description.fil
Fil: Lepanto, Paola. Instituto Pasteur de Montevideo; Uruguay  
dc.description.fil
Fil: Lecumberry, Federico. Universidad de la República. Facultad de Ciencias; Uruguay  
dc.description.fil
Fil: Rossello, Jéssica. Instituto Pasteur de Montevideo; Uruguay  
dc.description.fil
Fil: Kierbel, Arlinet Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.journal.title
Molecular And Cellular Probes  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mcp.2013.10.001  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0890850813000534