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dc.contributor.author
Vasen, Gustavo
dc.contributor.author
Battistone, Maria Agustina
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Croci Russo, Diego Omar
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Brukman, Nicolás Gastón
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Weigel Muñoz, Mariana
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Stupirski, Juan Carlos
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Rabinovich, Gabriel Adrian
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Cuasnicu, Patricia Sara
dc.date.available
2015-12-22T20:49:48Z
dc.date.issued
2015-07
dc.identifier.citation
Vasen, Gustavo; Battistone, Maria Agustina; Croci Russo, Diego Omar; Brukman, Nicolás Gastón; Weigel Muñoz, Mariana; et al.; The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization; Federation Amer Soc Exp Biol; Faseb Journal; 29; 10; 7-2015; 4189-4200
dc.identifier.issn
0892-6638
dc.identifier.uri
http://hdl.handle.net/11336/3189
dc.description.abstract
Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. Here, we identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal-1 resulted in glycan-dependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal-1-deficient (Lgals1-/-) mice revealed the essential contribution of this lectin for full sperm fertilizing ability both in vitro and in vivo. Mechanistically, Lgals1-/- sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, Lgals1-/- sperm showed a decreased ability to control cell volume and to undergo progesterone-induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal-1. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal-1-glycan axis in sperm function with critical implications in mammalian reproductive biology.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Federation Amer Soc Exp Biol
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Acrosome Reaction
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Capacitation
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Fertilization
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Galectin-1
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Glicosylation
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Motility
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Sperm
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Biología Reproductiva
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Otras Medicina Básica
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1530-6860
dc.journal.volume
29
dc.journal.number
10
dc.journal.pagination
4189-4200
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
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Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
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Fil: Croci Russo, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
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Fil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
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Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Stupirski, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires; Argentina; Argentina
dc.description.fil
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Faseb Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1096/fj.15-270975
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info:eu-repo/semantics/altIdentifier/url/http://www.fasebj.org/content/29/10/4189.abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1096/fj.15-270975