Artículo
The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
Vasen, Gustavo
; Battistone, Maria Agustina
; Croci Russo, Diego Omar
; Brukman, Nicolás Gastón
; Weigel Muñoz, Mariana
; Stupirski, Juan Carlos
; Rabinovich, Gabriel Adrián
; Cuasnicu, Patricia Sara
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Fecha de publicación:
07/2015
Editorial:
Federation Amer Soc Exp Biol
Revista:
Faseb Journal
ISSN:
0892-6638
e-ISSN:
1530-6860
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. Here, we identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal-1 resulted in glycan-dependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal-1-deficient (Lgals1-/-) mice revealed the essential contribution of this lectin for full sperm fertilizing ability both in vitro and in vivo. Mechanistically, Lgals1-/- sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, Lgals1-/- sperm showed a decreased ability to control cell volume and to undergo progesterone-induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal-1. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal-1-glycan axis in sperm function with critical implications in mammalian reproductive biology.
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Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Vasen, Gustavo; Battistone, Maria Agustina; Croci Russo, Diego Omar; Brukman, Nicolás Gastón; Weigel Muñoz, Mariana; et al.; The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization; Federation Amer Soc Exp Biol; Faseb Journal; 29; 10; 7-2015; 4189-4200
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