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dc.contributor.author
Daoust, Alexia
dc.contributor.author
Saoudi, Yasmina
dc.contributor.author
Brocard, Jacques
dc.contributor.author
Collomb, Nora
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Batandier, Cecile
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Bisbal, Mariano
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Salomé, Murielle
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Andrieux, Annie
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Bohic, Sylvain
dc.contributor.author
Barbier , Emmanuel
dc.date.available
2017-12-27T14:15:43Z
dc.date.issued
2014-05
dc.identifier.citation
Barbier , Emmanuel; Andrieux, Annie; Salomé, Murielle; Bisbal, Mariano; Batandier, Cecile; Collomb, Nora; et al.; Impact of manganese on primary hippocampal neurons from rodents; Wiley-liss, Div John Wiley & Sons Inc; Hippocampus; 24; 5; 5-2014; 598-610
dc.identifier.issn
1050-9631
dc.identifier.uri
http://hdl.handle.net/11336/31630
dc.description.abstract
Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn2+ may be toxic at high levels. In this study, we addressed the impact of Mn2+ on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn2+ leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn2+ in both cell types exposed to Mn2+ concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn2+ had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn2+ below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn2+ also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn2+ demonstrated here supports their use as a relevant model to study Mn2+-induced neurotoxicity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Manganese
dc.subject
Memri
dc.subject
X-Ray Synchrotron
dc.subject
Hippocampalneurons
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Mitochondria
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Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Impact of manganese on primary hippocampal neurons from rodents
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-26T20:39:13Z
dc.journal.volume
24
dc.journal.number
5
dc.journal.pagination
598-610
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Daoust, Alexia. Inserm; Francia. Université Grenoble Alpes. Grenoble; Francia
dc.description.fil
Fil: Saoudi, Yasmina. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
dc.description.fil
Fil: Brocard, Jacques. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
dc.description.fil
Fil: Collomb, Nora. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
dc.description.fil
Fil: Batandier, Cecile. Laboratoire de Bioénergétique. Grenoble; Francia
dc.description.fil
Fil: Bisbal, Mariano. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Salomé, Murielle. European Synchrotron Radiation Facility. Grenoble; Francia
dc.description.fil
Fil: Andrieux, Annie. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
dc.description.fil
Fil: Bohic, Sylvain. European Synchrotron Radiation Facility. Grenoble; Francia. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
dc.description.fil
Fil: Barbier , Emmanuel. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
dc.journal.title
Hippocampus
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/hipo.22252
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22252/abstract
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