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Artículo

Impact of manganese on primary hippocampal neurons from rodents

Daoust, Alexia; Saoudi, Yasmina; Brocard, Jacques; Collomb, Nora; Batandier, Cecile; Bisbal, MarianoIcon ; Salomé, Murielle; Andrieux, Annie; Bohic, Sylvain; Barbier , Emmanuel
Fecha de publicación: 05/2014
Editorial: Wiley-liss, Div John Wiley & Sons Inc
Revista: Hippocampus
ISSN: 1050-9631
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn2+ may be toxic at high levels. In this study, we addressed the impact of Mn2+ on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn2+ leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn2+ in both cell types exposed to Mn2+ concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn2+ had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn2+ below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn2+ also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn2+ demonstrated here supports their use as a relevant model to study Mn2+-induced neurotoxicity.
Palabras clave: Manganese , Memri , X-Ray Synchrotron , Hippocampalneurons , Mitochondria
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/31630
DOI: http://dx.doi.org/10.1002/hipo.22252
URL: http://onlinelibrary.wiley.com/doi/10.1002/hipo.22252/abstract
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Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Barbier , Emmanuel; Andrieux, Annie; Salomé, Murielle; Bisbal, Mariano; Batandier, Cecile; Collomb, Nora; et al.; Impact of manganese on primary hippocampal neurons from rodents; Wiley-liss, Div John Wiley & Sons Inc; Hippocampus; 24; 5; 5-2014; 598-610
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