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Artículo

Identification of phospholipase activity in Rhinella arenarum sperm extract capable of inducing oocyte activation

Bonilla, Eduardo Federico; Minahk, Carlos JavierIcon ; Ajmat, María Teresa; Sanchez Toranzo, Graciela; Bühler, Marta Inés
Fecha de publicación: 09/2013
Editorial: Cambridge University Press
Revista: Zygote
ISSN: 0967-1994
e-ISSN: 1469-8730
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Egg activation, which includes cortical granule exocytosis, resumption and completion of meiosis and pronuclear formation culminates in the first mitotic cleavage. However, the mechanism through which the fertilizing sperm induces this phenomenon is still controversial. We investigated the effect of the microinjection of homologous sperm soluble fractions obtained by fast protein liquid chromatography (FPLC) from reacted sperm (without acrosome) and non-reacted sperm on the activation of Rhinella arenarum oocytes matured in vitro. The FPLC-purified sperm fraction obtained from reacted or non-reacted sperm is able to induce oocyte activation when it is microinjected. This fraction has a 24 kDa protein and showed phospholipase C (PLC) activity in vitro, which was inhibited by D-609 but not by n-butanol or neomycin, suggesting that it is a PLC that is specific for phosphatidylcholine (PC-PLC). The assays conducted using inhibitors of inositol triphosphate (IP3) and ryanodine receptors (RyRs) indicate that the fraction with biological activity would act mainly through the cADPr (cyclic ADP ribose) pathway. Moreover, protein kinase C (PKC) inhibition blocks the activation produced by the same fraction. Immunocytochemical studies indicate that this PC-PLC can be found throughout the sperm head.
Palabras clave: Diacylglycerol , Oocyte Activation , Pc-Plc , Rhinella Arenarum , Sperm Factor
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/30516
DOI: http://dx.doi.org/10.1017/S0967199413000348
URL: https://www.cambridge.org/core/journals/zygote/article/identification-of-phospho
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Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Bonilla, Eduardo Federico; Minahk, Carlos Javier; Ajmat, María Teresa; Sanchez Toranzo, Graciela; Bühler, Marta Inés; Identification of phospholipase activity in Rhinella arenarum sperm extract capable of inducing oocyte activation; Cambridge University Press; Zygote; 22; 4; 9-2013; 483-495
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