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dc.contributor.author
Barrio, Alejandra
dc.contributor.author
Mora, Maria Celia
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Ramos, Federico
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Moreno, Sonia
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Samson, Ruth
dc.contributor.author
Basombrio, Miguel Angel Manuel
dc.date.available
2017-12-07T18:29:15Z
dc.date.issued
2007-10
dc.identifier.citation
Barrio, Alejandra; Mora, Maria Celia; Ramos, Federico; Moreno, Sonia; Samson, Ruth; et al.; Use of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 77; 4; 10-2007; 636-639
dc.identifier.issn
0002-9637
dc.identifier.uri
http://hdl.handle.net/11336/29990
dc.description.abstract
We evaluated the polymerase chain reaction (PCR) for the diagnosis of endemic American Tegumentary Leishmaniasis in Salta, Argentina. Diverse Leishmania species, coexistence of mycotic and varicose ulcers, and high endemicity T. cruzi, represent diagnostic challenges in the region. We performed a simplified PCR using sensitive, generic primers on samples obtained by a non-invasive method. We tested different culture types and clinical specimens with other microorganisms that induce leishmaniasis-like lesions. The PCR had a sensitivity and specificity of 100%. Forty-five patients with presumptive leishmaniasis were compared to the PCR, smears, and the Montenegro skin test (MST). In the same population, the PCR had an increased sensitivity, detecting 25 of 45 cases compared with 16 of 45 for smears and had a higher sensitivity in detecting mucocutaneous lesions. Diagnosis by PCR was supported by clinical presentation, positive MST results, compatible epidemiology, and in some cases histopathologic results or isolation of parasites by culture. These findings indicate the convenience of incorporating this PCR into diagnostic strategies for detecting leishmaniasis in northern Argentina.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Tropical Medicine and Hygiene
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Polymerase Chain Reaction
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Leishmaniasis American
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K Dna
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Diagnostic
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Use of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-29T15:38:21Z
dc.identifier.eissn
1476-1645
dc.journal.volume
77
dc.journal.number
4
dc.journal.pagination
636-639
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Illinois
dc.description.fil
Fil: Barrio, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Mora, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Ramos, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Moreno, Sonia. Provincia de Salta. Ministerio de Salud Publica. Hospital del Milagro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Samson, Ruth. Provincia de Salta. Ministerio de Salud Pública. Hospital del Milagro; Argentina
dc.description.fil
Fil: Basombrio, Miguel Angel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.journal.title
American Journal of Tropical Medicine and Hygiene
dc.relation.isreferencedin
info:eu-repo/semantics/reference/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2007.77.636
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2007.77.636


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