Artículo
Use of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina
Barrio, Alejandra; Mora, Maria Celia
; Ramos, Federico
; Moreno, Sonia; Samson, Ruth; Basombrio, Miguel Angel Manuel
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Fecha de publicación:
10/2007
Editorial:
American Society of Tropical Medicine and Hygiene
Revista:
American Journal of Tropical Medicine and Hygiene
ISSN:
0002-9637
e-ISSN:
1476-1645
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We evaluated the polymerase chain reaction (PCR) for the diagnosis of endemic American Tegumentary Leishmaniasis in Salta, Argentina. Diverse Leishmania species, coexistence of mycotic and varicose ulcers, and high endemicity T. cruzi, represent diagnostic challenges in the region. We performed a simplified PCR using sensitive, generic primers on samples obtained by a non-invasive method. We tested different culture types and clinical specimens with other microorganisms that induce leishmaniasis-like lesions. The PCR had a sensitivity and specificity of 100%. Forty-five patients with presumptive leishmaniasis were compared to the PCR, smears, and the Montenegro skin test (MST). In the same population, the PCR had an increased sensitivity, detecting 25 of 45 cases compared with 16 of 45 for smears and had a higher sensitivity in detecting mucocutaneous lesions. Diagnosis by PCR was supported by clinical presentation, positive MST results, compatible epidemiology, and in some cases histopathologic results or isolation of parasites by culture. These findings indicate the convenience of incorporating this PCR into diagnostic strategies for detecting leishmaniasis in northern Argentina.
Palabras clave:
Polymerase Chain Reaction
,
Leishmaniasis American
,
K Dna
,
Diagnostic
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Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Citación
Barrio, Alejandra; Mora, Maria Celia; Ramos, Federico; Moreno, Sonia; Samson, Ruth; et al.; Use of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 77; 4; 10-2007; 636-639
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