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Artículo

Evaluation of a Streptococcus thermophilus strain with innate anti-inflammatory properties as a vehicle for IL-10 cDNA delivery in an acute colitis model

del Carmen, Silvina AndreaIcon ; Miyoshi, Anderson; Azevedo, Vasco; de Moreno, Maria AlejandraIcon ; Leblanc, Jean Guy JosephIcon
Fecha de publicación: 20/02/2015
Editorial: Elsevier
Revista: Cytokine
ISSN: 1043-4666
e-ISSN: 1096-0023
Idioma: Inglés
Tipo de recurso: Artículo publicado

Resumen

The aim of this work was to develop a Streptococcus (S.) thermophilus strain with improved anti-inflammatory properties due to the incorporation of the therapeutic cDNA delivery plasmid pValac::il-10. To achieve this purpose, cells of S. thermophilus CRL807, previously selected as being an important anti-inflammatory strain, were electroporated with pValac::il-10 plasmid. In order to confirm the functionality of the developed strain, it was co-cultured with human epithelial cells Caco-2 and the production of IL-10 was evaluated by ELISA. Bacterial suspensions of S. thermophilus CRL807 containing pValac::il-10  plasmid or of the wild-type (WT) strain were administered in vivo using a murine model of intestinal inflammation. The animals treated with S. thermophilus CRL807 pValac::il-10 showed a lower body weight loss, microbial translocation to liver and damage scores in their intestines at macroscopical and microscopic levels. Furthermore, a significant increase was observed in the concentration of IL-10 in the intestinal contents of these mice compared to the rest of the experimental groups, accompanied by decreased levels of pro-inflammatory cytokines. The insertion of the therapeutic pValac::il-10  plasmid increased the intrinsic anti-inflammatory activity (synergetic effect) of S. thermophilus CRL807 which could be included in novel treatment protocols for inflammatory bowel diseases.
Palabras clave: Inflammatory Bowel Disease , Streptococcus Thermophils , Interleukin-10 , Dna Delivery , Anti Inflammatory
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/28420
DOI: http://dx.doi.org/10.1016/j.cyto.2015.02.020
URL: http://www.sciencedirect.com/science/article/pii/S1043466615000794
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Articulos(CERELA) [925]
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Citación
del Carmen, Silvina Andrea; Miyoshi, Anderson; Azevedo, Vasco; de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph; Evaluation of a Streptococcus thermophilus strain with innate anti-inflammatory properties as a vehicle for IL-10 cDNA delivery in an acute colitis model; Elsevier; Cytokine; 73; 2; 20-2-2015; 177-183
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