Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology

Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different depth of coverage per locus and can be exploited to model and non-model plant species.

Palabras clave: Reduced representation libraries , Next generation sequencing , RADseq , Genotyping by sequencing , SNP , SSR , InDels , Molecular markers

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/274737

DOI: https://doi.org/10.1007/978-1-0716-3024-2_4

URL: https://link.springer.com/protocol/10.1007/978-1-0716-3024-2_4

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Capítulos de libros (IABIMO)
Capítulos de libros de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR

Citación

Aguirre, Natalia Cristina; Filippi, Carla Valeria; Vera, Pablo Alfredo; Puebla, Andrea Fabiana; Zaina, Giusi; et al.; Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology; Humana Press; 2638; 2023; 37-57

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