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dc.contributor.author Chiesa, Ignacio Javier
dc.contributor.author Castillo, Lilian Fedra
dc.contributor.author Luthy, Isabel Alicia
dc.date.available 2017-10-03T16:49:43Z
dc.date.issued 2008
dc.identifier.citation Chiesa, Ignacio Javier; Castillo, Lilian Fedra; Luthy, Isabel Alicia; Contribution of alpha(2)-adrenoceptors to the mitogenic effect of catecholestrogen in human breast cancer MCF-7 cells; Elsevier; Journal of Steroid Biochemistry and Molecular Biology; 110; 1-2; -1-2008; 170-175
dc.identifier.issn 0960-0760
dc.identifier.uri http://hdl.handle.net/11336/25750
dc.description.abstract Catecholestrogens are estrogen metabolites formed by hydroxylation of 17beta-estradiol and estrone at either the C-2 or C-4 position, rivaling the parent estrogens in concentration. The objective of the present work was to assess if their catechol group could make them induce proliferation of human breast cancer cells via alpha(2)-adrenoceptors. In competition studies in human breast cancer MCF-7 cells, high concentrations of 2-hydroxy-estradiol (2-OH-E(2)), 2-hydroxy-estrone (2-OH-E(1)) and 4-hydroxy-estrone (4-OH-E(1)) competed for [(3)H]-rauwolscine binding, whereas 4-hydroxy-estradiol (4-OH-E(2)) did not. The contribution of alpha(2)-adrenoceptors and estrogen receptors (ERs) in proliferation enhancement was analyzed with specific antagonists. The specific alpha(2)-adrenergic antagonist yohimbine partially reversed the effect of catecholestrogens except 4-OH-E(2). The selective ER downregulator ICI-182780 or fulvestrant partially or totally reversed the effect of all hydroxylated catecholestrogens. When analyzing the effect of the combination of both antagonists in MCF-7, the contribution of the alpha(2)-adrenoceptors and ERs for 2-OH-E(2), 2-OH-E(1) and 4-OH-E(1) was mixed, whereas for 4-OH-E(2), the only receptor implied was an ER. In MDA-MB-231 cells (ER-alpha negative) the proliferation stimulation by these three catecholestrogens and reversal by the adrenergic antagonist was also observed. It can be concluded that alpha(2)-adrenoceptors contribute at least in part to the mitogenic effect of 2-OH-E(2), 2-OH-E(1) and 4-OH-E(1).
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ALPHA2 ADRENOCEPTORS
dc.subject CATECHOLESTROGEN
dc.subject BREAST CANCER
dc.subject ESTROGEN RECEPTORS
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Contribution of alpha(2)-adrenoceptors to the mitogenic effect of catecholestrogen in human breast cancer MCF-7 cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-25T18:31:59Z
dc.identifier.eissn 1879-1220
dc.journal.volume 110
dc.journal.number 1-2
dc.journal.pagination 170-175
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Chiesa, Ignacio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Castillo, Lilian Fedra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title Journal of Steroid Biochemistry and Molecular Biology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076008000848
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/ 10.1016/j.jsbmb.2008.03.035
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/18486470


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