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Artículo

Contribution of alpha(2)-adrenoceptors to the mitogenic effect of catecholestrogen in human breast cancer MCF-7 cells

Chiesa, Ignacio Javier; Castillo, Lilian FedraIcon ; Luthy, Isabel AliciaIcon
Fecha de publicación: 2008
Editorial: Elsevier
Revista: Journal of Steroid Biochemistry and Molecular Biology
ISSN: 0960-0760
e-ISSN: 1879-1220
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Catecholestrogens are estrogen metabolites formed by hydroxylation of 17beta-estradiol and estrone at either the C-2 or C-4 position, rivaling the parent estrogens in concentration. The objective of the present work was to assess if their catechol group could make them induce proliferation of human breast cancer cells via alpha(2)-adrenoceptors. In competition studies in human breast cancer MCF-7 cells, high concentrations of 2-hydroxy-estradiol (2-OH-E(2)), 2-hydroxy-estrone (2-OH-E(1)) and 4-hydroxy-estrone (4-OH-E(1)) competed for [(3)H]-rauwolscine binding, whereas 4-hydroxy-estradiol (4-OH-E(2)) did not. The contribution of alpha(2)-adrenoceptors and estrogen receptors (ERs) in proliferation enhancement was analyzed with specific antagonists. The specific alpha(2)-adrenergic antagonist yohimbine partially reversed the effect of catecholestrogens except 4-OH-E(2). The selective ER downregulator ICI-182780 or fulvestrant partially or totally reversed the effect of all hydroxylated catecholestrogens. When analyzing the effect of the combination of both antagonists in MCF-7, the contribution of the alpha(2)-adrenoceptors and ERs for 2-OH-E(2), 2-OH-E(1) and 4-OH-E(1) was mixed, whereas for 4-OH-E(2), the only receptor implied was an ER. In MDA-MB-231 cells (ER-alpha negative) the proliferation stimulation by these three catecholestrogens and reversal by the adrenergic antagonist was also observed. It can be concluded that alpha(2)-adrenoceptors contribute at least in part to the mitogenic effect of 2-OH-E(2), 2-OH-E(1) and 4-OH-E(1).
Palabras clave: Alpha2 Adrenoceptors , Catecholestrogen , Breast Cancer , Estrogen Receptors
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/25750
URL: http://www.sciencedirect.com/science/article/pii/S0960076008000848
DOI: http://dx.doi.org/ 10.1016/j.jsbmb.2008.03.035
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Chiesa, Ignacio Javier; Castillo, Lilian Fedra; Luthy, Isabel Alicia; Contribution of alpha(2)-adrenoceptors to the mitogenic effect of catecholestrogen in human breast cancer MCF-7 cells; Elsevier; Journal of Steroid Biochemistry and Molecular Biology; 110; 1-2; -1-2008; 170-175
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