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dc.contributor.author
Cara, Nicolás  
dc.contributor.author
Marfil, Carlos Federico  
dc.contributor.author
Garcia Lampasona, Sandra Claudia  
dc.contributor.author
Masuelli, Ricardo Williams  
dc.date.available
2025-03-12T11:46:03Z  
dc.date.issued
2014-06  
dc.identifier.citation
Cara, Nicolás; Marfil, Carlos Federico; Garcia Lampasona, Sandra Claudia; Masuelli, Ricardo Williams; Comparison of two detection systems to reveal AFLP markers in plants; National Research Council Canada-NRC Research Press; Botany; 92; 8; 6-2014; 607-610  
dc.identifier.issn
1916-2790  
dc.identifier.uri
http://hdl.handle.net/11336/256014  
dc.description.abstract
Since their development, AFLP (Amplified Fragment Length Polymorphism) markers have been used for a wide variety of analyses and, up to this day, are considered highly informative, robust and reproducible molecular markers. Originally, the visualization of the amplified fragments was done in polyacrylamide gels, followed by silver staining or by developing in an X-Ray plate, when radioactivity is used. In the last years, capillary electrophoresis of fluorescently labeled fragments is gradually replacing gel-based systems. However, the latter continue to be better for isolating and cloning AFLP fragments. In this report we contrast the results obtained by capillary electrophoresis against silver staining. We found that if fluorescence-labeled amplification products are loaded in a polyacrylamide gel, duplicated bands (doublets) are seen. This phenomenon is probably due to a delay in the migration of the strand which carries the fluorophore. Therefore, we recommend a minimum separation of 4 bp from the nearest fragment to the target fragment for its unambiguous identification and isolation. If this requirement is not fulfilled, an alternative is to make new amplifications using the same primer combination, but with unlabeled primers.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
National Research Council Canada-NRC Research Press  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
AFLP  
dc.subject
CAPILLARY ELECTROPHORESIS  
dc.subject
, SILVER STAINING  
dc.subject.classification
Ciencias de las Plantas, Botánica  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Comparison of two detection systems to reveal AFLP markers in plants  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2025-03-07T13:36:03Z  
dc.journal.volume
92  
dc.journal.number
8  
dc.journal.pagination
607-610  
dc.journal.pais
Canadá  
dc.journal.ciudad
Otawa  
dc.description.fil
Fil: Cara, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina  
dc.description.fil
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina  
dc.description.fil
Fil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina  
dc.description.fil
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina  
dc.journal.title
Botany  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/journal/cjb  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/cjb-2014-0047