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dc.contributor.author
Cara, Nicolás

dc.contributor.author
Marfil, Carlos Federico

dc.contributor.author
Garcia Lampasona, Sandra Claudia

dc.contributor.author
Masuelli, Ricardo Williams

dc.date.available
2025-03-12T11:46:03Z
dc.date.issued
2014-06
dc.identifier.citation
Cara, Nicolás; Marfil, Carlos Federico; Garcia Lampasona, Sandra Claudia; Masuelli, Ricardo Williams; Comparison of two detection systems to reveal AFLP markers in plants; National Research Council Canada-NRC Research Press; Botany; 92; 8; 6-2014; 607-610
dc.identifier.issn
1916-2790
dc.identifier.uri
http://hdl.handle.net/11336/256014
dc.description.abstract
Since their development, AFLP (Amplified Fragment Length Polymorphism) markers have been used for a wide variety of analyses and, up to this day, are considered highly informative, robust and reproducible molecular markers. Originally, the visualization of the amplified fragments was done in polyacrylamide gels, followed by silver staining or by developing in an X-Ray plate, when radioactivity is used. In the last years, capillary electrophoresis of fluorescently labeled fragments is gradually replacing gel-based systems. However, the latter continue to be better for isolating and cloning AFLP fragments. In this report we contrast the results obtained by capillary electrophoresis against silver staining. We found that if fluorescence-labeled amplification products are loaded in a polyacrylamide gel, duplicated bands (doublets) are seen. This phenomenon is probably due to a delay in the migration of the strand which carries the fluorophore. Therefore, we recommend a minimum separation of 4 bp from the nearest fragment to the target fragment for its unambiguous identification and isolation. If this requirement is not fulfilled, an alternative is to make new amplifications using the same primer combination, but with unlabeled primers.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Research Council Canada-NRC Research Press

dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AFLP
dc.subject
CAPILLARY ELECTROPHORESIS
dc.subject
, SILVER STAINING
dc.subject.classification
Ciencias de las Plantas, Botánica

dc.subject.classification
Ciencias Biológicas

dc.subject.classification
CIENCIAS NATURALES Y EXACTAS

dc.title
Comparison of two detection systems to reveal AFLP markers in plants
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2025-03-07T13:36:03Z
dc.journal.volume
92
dc.journal.number
8
dc.journal.pagination
607-610
dc.journal.pais
Canadá

dc.journal.ciudad
Otawa
dc.description.fil
Fil: Cara, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.fil
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.fil
Fil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.fil
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.journal.title
Botany

dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/journal/cjb
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/cjb-2014-0047
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