Artículo
Comparison of two detection systems to reveal AFLP markers in plants
Cara, Nicolás
; Marfil, Carlos Federico
; Garcia Lampasona, Sandra Claudia; Masuelli, Ricardo Williams



Fecha de publicación:
06/2014
Editorial:
National Research Council Canada-NRC Research Press
Revista:
Botany
ISSN:
1916-2790
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Since their development, AFLP (Amplified Fragment Length Polymorphism) markers have been used for a wide variety of analyses and, up to this day, are considered highly informative, robust and reproducible molecular markers. Originally, the visualization of the amplified fragments was done in polyacrylamide gels, followed by silver staining or by developing in an X-Ray plate, when radioactivity is used. In the last years, capillary electrophoresis of fluorescently labeled fragments is gradually replacing gel-based systems. However, the latter continue to be better for isolating and cloning AFLP fragments. In this report we contrast the results obtained by capillary electrophoresis against silver staining. We found that if fluorescence-labeled amplification products are loaded in a polyacrylamide gel, duplicated bands (doublets) are seen. This phenomenon is probably due to a delay in the migration of the strand which carries the fluorophore. Therefore, we recommend a minimum separation of 4 bp from the nearest fragment to the target fragment for its unambiguous identification and isolation. If this requirement is not fulfilled, an alternative is to make new amplifications using the same primer combination, but with unlabeled primers.
Palabras clave:
AFLP
,
CAPILLARY ELECTROPHORESIS
,
, SILVER STAINING
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Articulos(IBAM)
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Citación
Cara, Nicolás; Marfil, Carlos Federico; Garcia Lampasona, Sandra Claudia; Masuelli, Ricardo Williams; Comparison of two detection systems to reveal AFLP markers in plants; National Research Council Canada-NRC Research Press; Botany; 92; 8; 6-2014; 607-610
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