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dc.contributor.author Meikle, V.
dc.contributor.author Alito, A.
dc.contributor.author Llera, Andrea Sabina
dc.contributor.author Gioffré, Andrea Karina
dc.contributor.author Peralta, Andrea Verónica
dc.contributor.author Buddle, B. M.
dc.contributor.author Cataldi, Ángel Adrián
dc.date.available 2017-09-29T16:02:24Z
dc.date.issued 2009-09
dc.identifier.citation Meikle, V.; Alito, A.; Llera, Andrea Sabina; Gioffré, Andrea Karina; Peralta, Andrea Verónica; et al.; Identification of novel Mycobacterium bovis antigens by disection of crude protein fractions; American Society for Microbiology; Clinical And Vaccine Immunology; 16; 9; 9-2009; 1352-1359
dc.identifier.issn 1556-6811
dc.identifier.uri http://hdl.handle.net/11336/25423
dc.description.abstract Culture filtrate and cell extracts from Mycobacterium bovis cultures contain molecules which could promote protective immunity to tuberculosis in animals. Different protein fractions of M. bovis cultures were obtained by elution electrophoresis and were tested in experimentally infected cattle. The fractions that elicited gamma interferon (IFN-γ) responses were resolved by two-dimensional gel electrophoresis, and individual proteins were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The open reading frames were cloned, expressed as their recombinant forms, and retested with naturally and experimentally infected animals. Eleven protein fractions were highly reactive, from which the Rv1636, HspX, Rv0138, Rv2524, EsxI, and Rv3740 recombinant proteins were obtained. EsxI and HspX were the antigens most recognized by the IFN-γ release assay. In summary, a proteomic approach allowed the identification of novel antigens useful for the diagnosis of bovine tuberculosis.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject Mycobacterium bovis
dc.subject Antigens
dc.subject Discovery
dc.subject Proteomics
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Identification of novel Mycobacterium bovis antigens by disection of crude protein fractions
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-28T18:15:20Z
dc.journal.volume 16
dc.journal.number 9
dc.journal.pagination 1352-1359
dc.journal.pais Estados Unidos
dc.description.fil Fil: Meikle, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil Fil: Alito, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil Fil: Llera, Andrea Sabina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; Argentina
dc.description.fil Fil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil Fil: Buddle, B. M.. Hopkirk Research Institute. AgResearch; Nueva Zelanda
dc.description.fil Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Clinical And Vaccine Immunology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/CVI.00211-09
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://cvi.asm.org/content/16/9/1352.short


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)