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Artículo

Identification of novel Mycobacterium bovis antigens by disection of crude protein fractions

Meikle, V.; Alito, A.; Llera, Andrea SabinaIcon ; Gioffré, Andrea KarinaIcon ; Peralta, Andrea VerónicaIcon ; Buddle, B. M.; Cataldi, Ángel AdriánIcon
Fecha de publicación: 09/2009
Editorial: American Society for Microbiology
Revista: Clinical And Vaccine Immunology
ISSN: 1556-6811
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Culture filtrate and cell extracts from Mycobacterium bovis cultures contain molecules which could promote protective immunity to tuberculosis in animals. Different protein fractions of M. bovis cultures were obtained by elution electrophoresis and were tested in experimentally infected cattle. The fractions that elicited gamma interferon (IFN-γ) responses were resolved by two-dimensional gel electrophoresis, and individual proteins were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The open reading frames were cloned, expressed as their recombinant forms, and retested with naturally and experimentally infected animals. Eleven protein fractions were highly reactive, from which the Rv1636, HspX, Rv0138, Rv2524, EsxI, and Rv3740 recombinant proteins were obtained. EsxI and HspX were the antigens most recognized by the IFN-γ release assay. In summary, a proteomic approach allowed the identification of novel antigens useful for the diagnosis of bovine tuberculosis.
Palabras clave: Mycobacterium Bovis , Antigens , Discovery , Proteomics
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)
Identificadores
URI: http://hdl.handle.net/11336/25423
DOI: http://dx.doi.org/10.1128/CVI.00211-09
URL: http://cvi.asm.org/content/16/9/1352.short
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Citación
Meikle, V.; Alito, A.; Llera, Andrea Sabina; Gioffré, Andrea Karina; Peralta, Andrea Verónica; et al.; Identification of novel Mycobacterium bovis antigens by disection of crude protein fractions; American Society for Microbiology; Clinical And Vaccine Immunology; 16; 9; 9-2009; 1352-1359
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