Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences

Vera Candioti, LucianaIcon ; Culzoni, Maria JuliaIcon ; Olivieri, Alejandro CesarIcon ; Goicoechea, Hector CasimiroIcon
Fecha de publicación: 12/2008
Editorial: Wiley VCH Verlag
Revista: Electrophoresis
ISSN: 0173-0835
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

Drug monitoring in serum samples was performed using second-order data generated by CE-DAD, processed with a suitable chemometric strategy. Carbamazepine could be accurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additional<br />serum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and (iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, its metabolite, lamotrigine and unexpected serum components) by the well-known multivariate curve resolution-alternating least squares algorithm, which extracts quantitative information that can be uniquely ascribed to the analyte of interest. The analyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoveries<br />were 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracy and precision of the proposed method
Palabras clave: Carbamazepine , Overlapped Peaks , Second-Order Advantage
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 216.6Kb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/25385
DOI: http://dx.doi.org/10.1002/elps.200800400
URL: http://onlinelibrary.wiley.com/doi/10.1002/elps.200800400/abstract
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Vera Candioti, Luciana; Culzoni, Maria Julia; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences; Wiley VCH Verlag; Electrophoresis; 29; 22; 12-2008; 4527-4537
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES