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dc.contributor.author
Imperiale, Belén Rocío
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dc.contributor.author
Zumárraga, Martín José
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dc.contributor.author
Di Giulio, A. B.
dc.contributor.author
Cataldi, Ángel Adrián
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dc.contributor.author
Morcillo, Nora Susana
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dc.date.available
2017-09-27T20:47:07Z
dc.date.issued
2013-08
dc.identifier.citation
Imperiale, Belén Rocío; Zumárraga, Martín José; Di Giulio, A. B.; Cataldi, Ángel Adrián; Morcillo, Nora Susana; Molecular and phenotypic characterisation of Mycobacterium tuberculosis resistant to anti-tuberculosis drugs; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 17; 8; 8-2013; 1088-1093
dc.identifier.issn
1027-3719
dc.identifier.uri
http://hdl.handle.net/11336/25273
dc.description.abstract
SETTING: Dr Cetrángolo Hospital, Buenos Aires, Argentina. OBJECTIVES: To characterise drug-resistant (DR), multidrug-resistant (MDR-) and extensively drug-resistant (XDR-) Mycobacterium tuberculosis isolates, and identify their genetic profiles, drug resistance levels and resistance- conferring mutations. DESIGN: Phenotypic drug susceptibility testing methods were used to determine drug resistance profiles. Minimal inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RMP) and levofloxacin (LVX) from 169 DR tuberculosis (TB) isolates, 78 of them monoresistant to INH, 13 to RMP, 7 to LVX, and 71 MDR-TB, were determined. Multiplex allele-specific polymerase chain reaction and DNA sequencing were used to detect mutations in katG, rpoB and gyrA/B genes. Genotyping was performed using spoligotyping and insertion sequence 6110 restriction fragment length polymorphism. RESULTS: In total, 38.9% of the INH-resistant (INHR) isolates had an MIC ≥ 32 μg/ml; 61.3% of the RMP-resistant (RMPR) isolates had an MIC ≥ 64 μg/ml and 55.6% of the LVX-r esistant (LVXR) isolates had an MIC 4-≥16 μg/ml. The main mutations found in INHR isolates were katG315 (53.7%) and inhAP-15 (25.5%), whereas in RMPR isolates the main mutations were rpoB531 (61.9%), followed by rpoB526 (16.7%). LVX R isolates showed mutations in gyrA94/90. Haarlem, LAM and T were the main spoligotyping families found. katG315 was mainly associated with Haarlem and LAM, whereas inhAP-15 was associated with T. CONCLUSIONS: Several isolates showed an association between high INHR levels and katG mutation; others from the Haarlem family were prone to becoming MDR-TB and continue to circulate in the community.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
International Union Against Tuberculosis and Lung Disease
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Drug Resistance Levels
dc.subject
Genetic Mutations
dc.subject
Genotypes
dc.subject
M. Tuberculosis
dc.subject.classification
Enfermedades Infecciosas
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Molecular and phenotypic characterisation of Mycobacterium tuberculosis resistant to anti-tuberculosis drugs
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-21T18:55:02Z
dc.journal.volume
17
dc.journal.number
8
dc.journal.pagination
1088-1093
dc.journal.pais
Francia
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dc.journal.ciudad
París
dc.description.fil
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.description.fil
Fil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Di Giulio, A. B.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Zonal General Agudos "Petrona V. De Cordero"; Argentina
dc.description.fil
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Morcillo, Nora. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.journal.title
International Journal of Tuberculosis and Lung Disease
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/iuatld/ijtld/2013/00000017/00000008/art00017
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.5588/ijtld.12.0949
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