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Artículo

Molecular and phenotypic characterisation of Mycobacterium tuberculosis resistant to anti-tuberculosis drugs

Imperiale, Belén RocíoIcon ; Zumárraga, Martín JoséIcon ; Di Giulio, A. B.; Cataldi, Ángel AdriánIcon ; Morcillo, Nora Susana
Fecha de publicación: 08/2013
Editorial: International Union Against Tuberculosis and Lung Disease
Revista: International Journal of Tuberculosis and Lung Disease
ISSN: 1027-3719
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Enfermedades Infecciosas

Resumen

SETTING: Dr Cetrángolo Hospital, Buenos Aires, Argentina. OBJECTIVES: To characterise drug-resistant (DR), multidrug-resistant (MDR-) and extensively drug-resistant (XDR-) Mycobacterium tuberculosis isolates, and identify their genetic profiles, drug resistance levels and resistance- conferring mutations. DESIGN: Phenotypic drug susceptibility testing methods were used to determine drug resistance profiles. Minimal inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RMP) and levofloxacin (LVX) from 169 DR tuberculosis (TB) isolates, 78 of them monoresistant to INH, 13 to RMP, 7 to LVX, and 71 MDR-TB, were determined. Multiplex allele-specific polymerase chain reaction and DNA sequencing were used to detect mutations in katG, rpoB and gyrA/B genes. Genotyping was performed using spoligotyping and insertion sequence 6110 restriction fragment length polymorphism. RESULTS: In total, 38.9% of the INH-resistant (INHR) isolates had an MIC ≥ 32 μg/ml; 61.3% of the RMP-resistant (RMPR) isolates had an MIC ≥ 64 μg/ml and 55.6% of the LVX-r esistant (LVXR) isolates had an MIC 4-≥16 μg/ml. The main mutations found in INHR isolates were katG315 (53.7%) and inhAP-15 (25.5%), whereas in RMPR isolates the main mutations were rpoB531 (61.9%), followed by rpoB526 (16.7%). LVX R isolates showed mutations in gyrA94/90. Haarlem, LAM and T were the main spoligotyping families found. katG315 was mainly associated with Haarlem and LAM, whereas inhAP-15 was associated with T. CONCLUSIONS: Several isolates showed an association between high INHR levels and katG mutation; others from the Haarlem family were prone to becoming MDR-TB and continue to circulate in the community.
Palabras clave: Drug Resistance Levels , Genetic Mutations , Genotypes , M. Tuberculosis
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/25273
URL: http://www.ingentaconnect.com/content/iuatld/ijtld/2013/00000017/00000008/art000
DOI: http://dx.doi.org/10.5588/ijtld.12.0949
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Imperiale, Belén Rocío; Zumárraga, Martín José; Di Giulio, A. B.; Cataldi, Ángel Adrián; Morcillo, Nora Susana; Molecular and phenotypic characterisation of Mycobacterium tuberculosis resistant to anti-tuberculosis drugs; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 17; 8; 8-2013; 1088-1093
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