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dc.contributor.author
Bovi, Michele
dc.contributor.author
Cenci, Lucia
dc.contributor.author
Perduca, Massimiliano
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Capaldi, Stefano
dc.contributor.author
Carrizo Garcia, Maria Elena
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dc.contributor.author
Civiero, Laura
dc.contributor.author
Chiarelli, Laurent R.
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Galliano, Monica
dc.contributor.author
Monaco, Hugo L.
dc.date.available
2017-09-22T19:18:01Z
dc.date.issued
2012-12
dc.identifier.citation
Bovi, Michele; Cenci, Lucia; Perduca, Massimiliano; Capaldi, Stefano; Carrizo Garcia, Maria Elena; et al.; BEL β-trefoil: A novel lectin with antineoplastic properties in king bolete (Boletus edulis) mushrooms; Oxford University Press; Glycobiology; 23; 5; 12-2012; 578-592
dc.identifier.issn
0959-6658
dc.identifier.uri
http://hdl.handle.net/11336/24989
dc.description.abstract
A novel lectin was purified from the fruiting bodies of king bolete mushrooms (Boletus edulis, also called porcino, cep or penny bun). The lectin was structurally characterized i.e its amino acid sequence and three-dimensional structure were determined. The new protein is a homodimer and each protomer folds as β-trefoil domain and therefore we propose the name Boletus edulis lectin (BEL) β-trefoil to distinguish it from the other lectin that has been described in these mushrooms. The lectin has potent anti-proliferative effects on human cancer cells, which confers to it an interesting therapeutic potential as an antineoplastic agent. Several crystal forms of the apoprotein and of complexes with different carbohydrates were studied by X-ray diffraction. The structure of the apoprotein was solved at 1.12 Å resolution. The interaction of the lectin with lactose, galactose, N-acetylgalactosamine and T-antigen disaccharide, Galβ1-3GalNAc, was examined in detail. All the three potential binding sites present in the β-trefoil fold are occupied in at least one crystal form and are described in detail in this paper. No important conformational changes are observed in the lectin when comparing its co-crystals with carbohydrates with those of the ligand-free protein.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Boletus Edulis
dc.subject
Lectin
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Beta Trefoil
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Crystal Structure
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
BEL β-trefoil: A novel lectin with antineoplastic properties in king bolete (Boletus edulis) mushrooms
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-30T13:54:13Z
dc.journal.volume
23
dc.journal.number
5
dc.journal.pagination
578-592
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Oxford
dc.description.fil
Fil: Bovi, Michele. Universita Di Verona; Italia
dc.description.fil
Fil: Cenci, Lucia. Universita Di Verona; Italia
dc.description.fil
Fil: Perduca, Massimiliano. Universita Di Verona; Italia
dc.description.fil
Fil: Capaldi, Stefano. Universita Di Verona; Italia
dc.description.fil
Fil: Carrizo Garcia, Maria Elena. Universidad Catolica de Córdoba. Facultad de Medicina. Departamento de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Civiero, Laura. Università di Padova; Italia
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Fil: Chiarelli, Laurent R.. Università di Pavia; Italia
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Fil: Galliano, Monica. Università di Pavia; Italia
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Fil: Monaco, Hugo L.. Universita Di Verona; Italia
dc.journal.title
Glycobiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/glycob/cws164
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article-lookup/doi/10.1093/glycob/cws164
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