Artículo
BEL β-trefoil: A novel lectin with antineoplastic properties in king bolete (Boletus edulis) mushrooms
Bovi, Michele; Cenci, Lucia; Perduca, Massimiliano; Capaldi, Stefano; Carrizo Garcia, Maria Elena
; Civiero, Laura; Chiarelli, Laurent R.; Galliano, Monica; Monaco, Hugo L.
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
12/2012
Editorial:
Oxford University Press
Revista:
Glycobiology
ISSN:
0959-6658
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A novel lectin was purified from the fruiting bodies of king bolete mushrooms (Boletus edulis, also called porcino, cep or penny bun). The lectin was structurally characterized i.e its amino acid sequence and three-dimensional structure were determined. The new protein is a homodimer and each protomer folds as β-trefoil domain and therefore we propose the name Boletus edulis lectin (BEL) β-trefoil to distinguish it from the other lectin that has been described in these mushrooms. The lectin has potent anti-proliferative effects on human cancer cells, which confers to it an interesting therapeutic potential as an antineoplastic agent. Several crystal forms of the apoprotein and of complexes with different carbohydrates were studied by X-ray diffraction. The structure of the apoprotein was solved at 1.12 Å resolution. The interaction of the lectin with lactose, galactose, N-acetylgalactosamine and T-antigen disaccharide, Galβ1-3GalNAc, was examined in detail. All the three potential binding sites present in the β-trefoil fold are occupied in at least one crystal form and are described in detail in this paper. No important conformational changes are observed in the lectin when comparing its co-crystals with carbohydrates with those of the ligand-free protein.
Palabras clave:
Boletus Edulis
,
Lectin
,
Beta Trefoil
,
Crystal Structure
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Identificadores
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Bovi, Michele; Cenci, Lucia; Perduca, Massimiliano; Capaldi, Stefano; Carrizo Garcia, Maria Elena; et al.; BEL β-trefoil: A novel lectin with antineoplastic properties in king bolete (Boletus edulis) mushrooms; Oxford University Press; Glycobiology; 23; 5; 12-2012; 578-592
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